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Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
Published on: February 8, 2020
Circ_0039569 promotes renal cell carcinoma growth and metastasis by regulating miR-34a-5p/CCL22
Chengluo Jin1, Linmei Shi2, Zhexun Li1
1Department of Urology, The Second Affiliate Hospital, Harbin Medical University Harbin, China.
Abstract:
Circular RNAs (circRNAs) belong to non-coding RNAs and are known as key regulators in gene regulation. CircRNAs involve in the various biological processes of cancer. However, the functions of circRNAs in renal cell carcinoma (RCC) are still not clear. In this study, the circRNA expression profile was performed in the RCC tissues by microarray. There were 35 significantly expressed circRNAs with more than 5 folds from microarray analysis. Hsa_circ_0039569 (circ_0039569) was verified to be up-regulated in RCC and cells compared with the controls by real time RT-PCR. The assays of cellular functions showed that circ_0039569 down-regulation suppressed the proliferation and metastasis of RCC cells. The molecular mechanism of circ_0039569 in RCC cells showed that circ_0039569 promoted RCC progression by up-regulating CCL22 expression via down-regulating miR-34a-5p. Taken together, the study indicated that circ_0039569 played an important role in RCC cell survival and metastasis, which suggested that an oncogenic role of circ_0039569 in RCC progression.
Insights
Circular RNAs (circRNAs) are key gene regulators in cancer. This study found that circ_0039569 promotes renal cell carcinoma (RCC) progression and metastasis by regulating miR-34a-5p and CCL22.
Area of Science:
- Oncology
- Molecular Biology
- Non-coding RNA Research
Background:
- Circular RNAs (circRNAs) are non-coding RNAs regulating gene expression.
- The role of circRNAs in renal cell carcinoma (RCC) remains largely uncharacterized.
- circRNAs are implicated in various cancer biological processes.
Purpose of the Study:
- To investigate the function and mechanism of circRNAs in renal cell carcinoma (RCC).
- To identify specific circRNAs involved in RCC progression.
- To elucidate the role of Hsa_circ_0039569 in RCC cell survival and metastasis.
Main Methods:
- Microarray analysis of circRNA expression profiles in RCC tissues.
- Real-time RT-PCR for verifying circRNA expression levels.
- Cellular function assays to assess proliferation and metastasis.
- Molecular mechanism investigation involving microRNAs and target genes.
Main Results:
- Identified 35 significantly expressed circRNAs in RCC tissues.
- Hsa_circ_0039569 (circ_0039569) was significantly upregulated in RCC.
- Downregulation of circ_0039569 suppressed RCC cell proliferation and metastasis.
- circ_0039569 promotes RCC progression by upregulating CCL22 via downregulating miR-34a-5p.
Conclusions:
- circ_0039569 plays a crucial role in RCC cell survival and metastasis.
- circ_0039569 exhibits an oncogenic role in renal cell carcinoma progression.
- circ_0039569 may serve as a potential therapeutic target for RCC.
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