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Related Concept Videos

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice16:48

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

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Two-photon imaging has uncovered lymphocyte motility and cellular interactions within the lymph node under basal conditions and durring an immune response 1. Here, we demonstrate adoptive transfer of T cells, isolation of lymph nodes, and imaging motility of CD4+ T cells in the explanted lymph...
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Lymph Node Exam08:06

Lymph Node Exam

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Source: Richard Glickman-Simon, MD, Assistant Professor, Department of Public Health and Community Medicine, Tufts University School of Medicine, MA
The lymphatic system has two main functions: to return extracellular fluid back to the venous circulation and to expose antigenic substances to the immune system. As the collected fluid passes through lymphatic channels on its way back to the systemic circulation, it encounters multiple nodes consisting of highly concentrated clusters of...
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Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin09:10

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

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Generation of lymph node/fat pad chimeras for the study of lymph node stromal cell origin is described. The method involves the isolation of lymph nodes from newborn mice and embryonic fat pads, the generation of chimeric lymph node-fat pads, and their transfer under the kidney capsule of a host...
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A Dissection Method to Harvest Murine Popliteal Draining Lymph Nodes for Ex Vivo Imaging02:16

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This video demonstrates a dissection technique to harvest popliteal draining lymph nodes from the mouse hind legs. Lymph nodes play a critical role in bridging innate and adaptive immunity, and harvesting intact lymph nodes — with a preserved tissue structure and cellular viability — can help study local and systemic immune...
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Intravital Microscopy of the Inguinal Lymph Node07:34

Intravital Microscopy of the Inguinal Lymph Node

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A technique for performing intravital microscopy of the inguinal lymph node (LN) is outlined. Such technique allows for real-time, in vivo study of the lymph node microvasculature and structure both during homeostasis and infection. This technique can be adapted to cell trafficking studies and to other lymph node...
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Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species12:53

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

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This protocol provides an overview of procedures for the isolation of RNA for the transcriptomic profiling of lymph node tissues from large animals, including steps in the identification and excision of lymph nodes from livestock and wildlife, sampling approaches to provide consistency across multiple animals, and considerations plus representative results for the post-collection preservation and processing for RNA...
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Related Experiment Video

Updated: Jan 19, 2026

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
16:48

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

Published on: August 23, 2007

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[Rationale of thoracic lymph node dissection in pulmonary metastasectomy].

S Sponholz1, M Schirren2, J Schirren2

  • 1Klinik für Thoraxchirurgie, Agaplesion Markus Krankenhaus, Wilhelm-Epstein-Str. 4, 60431, Frankfurt/M., Deutschland. stefan.sponholz@fdk.info.

Der Chirurg; Zeitschrift Fur Alle Gebiete Der Operativen Medizen
|September 11, 2019
PubMed
Summary

Systematic lymph node dissection in pulmonary metastasectomy is not standard, despite high metastasis prevalence. This review evaluates its rationale and impact on patient selection for lung cancer surgery.

Keywords:
Long-term survivalLymph node metastasesMetastasectomyOncological stagingPreoperative diagnostics

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Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
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Lymph Nodes: Head & Neck, Axillae, Upper & Lower Extremities
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Related Experiment Videos

Last Updated: Jan 19, 2026

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Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

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Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
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Lymph Nodes: Head & Neck, Axillae, Upper & Lower Extremities
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Area of Science:

  • Thoracic Surgery
  • Surgical Oncology
  • Pulmonary Medicine

Background:

  • Metastasectomy aims for R0 resection, but lymph node involvement in pulmonary metastases varies by tumor type, reaching up to 45%.
  • Systematic lymph node dissection is not a universally established component of pulmonary metastasectomy.
  • The clinical significance of lymph node status and its impact on surgical candidacy remains debated.

Purpose of the Study:

  • To review the current literature regarding the rationale for systematic lymph node dissection during pulmonary metastasectomy.
  • To determine if patients with thoracic lymph node metastases should be excluded from pulmonary metastasectomy.
  • To assess the long-term survival outcomes associated with lymph node-positive pulmonary metastasectomy.

Main Methods:

  • Literature review of studies on pulmonary metastasectomy and lymph node dissection.
  • Analysis of prevalence and prognostic significance of lymph node metastases.
  • Evaluation of patient selection criteria for pulmonary metastasectomy.

Main Results:

  • High prevalence of lymph node metastases in pulmonary metastasectomy, increasing with the number of lung lesions.
  • Uncertainty regarding the routine inclusion of systematic lymph node dissection.
  • Potential for good long-term survival even with positive lymph nodes, suggesting non-exclusion.

Conclusions:

  • The role of systematic lymph node dissection in pulmonary metastasectomy requires further clarification.
  • Excluding patients with thoracic lymph node metastases may be unwarranted, as survival can still be favorable.
  • Further research is needed to establish optimal surgical guidelines for pulmonary metastasectomy with lymph node involvement.