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Related Experiment Video

Updated: Jan 19, 2026

Using Primary Neurosphere Cultures to Study Primary Cilia
08:14

Using Primary Neurosphere Cultures to Study Primary Cilia

Published on: April 14, 2017

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Primary cilia have a length-dependent persistence length.

Justin Flaherty1, Zhe Feng2, Zhangli Peng3

  • 1Department of Physics, The Ohio State University, Columbus, USA.

Biomechanics and Modeling in Mechanobiology
|September 11, 2019
PubMed
Summary
This summary is machine-generated.

Cilia stiffness depends on their length, with longer cilia being stiffer. This finding, based on modeling and experiments, helps understand cellular flow sensing.

Keywords:
Elastic shellMechanobiologyPrimary cilia

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Related Experiment Videos

Last Updated: Jan 19, 2026

Using Primary Neurosphere Cultures to Study Primary Cilia
08:14

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Published on: April 14, 2017

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Simple Detection of Primary Cilia by Immunofluorescence
08:07

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Area of Science:

  • Cell Biology
  • Biophysics
  • Biomechanical Engineering

Background:

  • Cilia are crucial for cellular functions, including movement and sensing.
  • Understanding the mechanical properties of cilia is essential for comprehending their biological roles.

Purpose of the Study:

  • To characterize the mechanical properties of the cilium axoneme and basal body.
  • To investigate the length-dependent persistence length of ciliary axonemes.

Main Methods:

  • Utilized optical trapping to monitor cilium tip fluctuations.
  • Combined experimental, analytical, and computational modeling approaches.
  • Modeled axonemal microtubules as anisotropic elastic shells and included basal body motion.

Main Results:

  • Provided the first evidence of length-dependent persistence length in ciliary axonemes.
  • Demonstrated that longer cilia exhibit greater stiffness than shorter cilia.
  • Showed that actomyosin-driven basal body motion contributes to observed dynamics.

Conclusions:

  • Ciliary axoneme stiffness is dependent on its length.
  • Observable ciliary dynamics can be used to probe internal cytoskeletal dynamics.
  • Improved characterization of cilia enhances understanding of cellular flow sensing.