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3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
Published on: September 13, 2018
MiR-212-3p inhibits cell proliferation and promotes apoptosis by targeting nuclear factor IA in bladder cancer
Xiaoming Wu1, Hao Chen, Gaoyue Zhang
1Department of Urology, the First Hospital of Jiaxing, Jiaxing, Zhejiang Province, People's Republic of China.
Abstract:
Accumulating evidence suggest that microRNAs play crucial roles in the development and progression of bladder cancer (BC). Here, we found that miR-212-3p was significantly down-regulated and negatively correlated with nuclear factor IA (NFIA) in human BC tissues. Bioinformatics analysis predicted that NFIA was a target gene of miR-212-3p. Then BC cell lines, T24 and J82 cells were transfected with miR-212-3p mimics or siNFIA to obtain miR-212-3p overexpression or NFIA knockdown cell lines, respectively. Quantitative real-time PCR was used to determine the expression of miR-212-3p and NFIA. Western blot analysis was utilized to detect NFIA expression. MTT assay showed either miR-212-3 overexpression or NFIA knockdown significantly inhibited the BC cell proliferation. Double staining with Annexin V-APC and 7-AAD showed the total number of apoptotic BC cells were remarkably increased after miR-212-3p overexpression or NFIA knockdown. Collectively, our results indicated that miR-212-3p targeting NFIA might serve as a promising target for BC.
Insights
MicroRNA-212-3p is downregulated in bladder cancer (BC) and targets NFIA. Inhibiting NFIA or increasing miR-212-3p suppresses BC cell proliferation and promotes apoptosis, suggesting a potential therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNAs (miRNAs) are critical regulators in cancer development and progression.
- Bladder cancer (BC) is a significant global health concern with complex molecular underpinnings.
Purpose of the Study:
- To investigate the role of miR-212-3p in bladder cancer.
- To identify the molecular targets and mechanisms of miR-212-3p in BC.
Main Methods:
- Bioinformatic analysis to predict miRNA-target interactions.
- Quantitative real-time PCR and Western blot to assess gene and protein expression.
- Cell proliferation (MTT assay) and apoptosis (Annexin V/7-AAD staining) assays in BC cell lines.
Main Results:
- miR-212-3p was significantly downregulated in human BC tissues and negatively correlated with NFIA expression.
- NFIA was identified as a direct target gene of miR-212-3p.
- Overexpression of miR-212-3p or knockdown of NFIA inhibited BC cell proliferation and induced significant apoptosis.
Conclusions:
- miR-212-3p exerts tumor-suppressive functions in bladder cancer by targeting NFIA.
- The miR-212-3p/NFIA axis represents a potential therapeutic strategy for bladder cancer treatment.
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