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Updated: Jan 19, 2026

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy
Published on: November 29, 2017
Reflection confocal nanoscopy using a super-oscillatory lens
Abstract:
A superoscillatory lens (SOL) is known to produce a sub-diffraction hotspot that is useful for high-resolution imaging. SOLs have not yet been directly used in a confocal reflection setup, as the SOL suffers from poor imaging properties. Additionally, the illuminating intensity distribution of the SOL still has high-intensity rings called sidelobes coexisting with the central hotspot. By means of a reflection setup, which does not have the SOL in the detection chain, thereby mitigating the poor imaging properties, we assessed the resolution capabilities of a SOL. This was done for different objects, whose dimensions were both above and below the SOL field-of-view (FOV). We found that the sidelobe illumination degrades the imaging properties in the case of extended objects, limiting the applicability of a SOL system.
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Reflection and Refraction
Light travels at different speeds depending on the material through which it is propagating. When light travels from one material to another, it will either slow down or speed up. In order to conserve energy and momentum, the light must change the direction in which it propagates. This bending of light is known as refraction. Some fraction of...
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