Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy14:11

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

16.3K
This article describes a working protocol to image dendritic spines from hippocampal neurons in vitro using Structured Illumination Microscopy (SIM). Super-resolution microscopy using SIM provides image resolution significantly beyond the light diffraction limit in all three spatial dimensions, allowing the imaging of individual dendritic spines with improved...
16.3K
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

11.9K
Lensfree optical tomography is a three-dimensional microscopy technique that offers a spatial resolution of <1 μm × <1 μm × <3 μm in x, y and z dimensions, respectively, over a large imaging-volume of 15-100 mm3, which can be particularly useful for integration with lab-on-a-chip...
11.9K
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

26.1K
This article provides an in depth guide for the assembly and operation of a structured illumination microscope operating with total internal reflection fluorescence illumination (TIRF-SIM) to image dynamic biological processes with optical super-resolution in multiple...
26.1K
Imaging Biological Samples with Optical and Confocal Microscopy10:48

Imaging Biological Samples with Optical and Confocal Microscopy

37.6K
Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut
Optical microscopes have been around for centuries, and while they reached their theoretical limitation of resolution decades ago, new equipment and techniques, such as confocal and digital image processing, have created new niches within the field of optical imaging. The best optical microscopes will typically have a resolution down to 200 nm in ideal...
37.6K
Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy07:53

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

7.7K
The protocol describes specific labeling of mitochondrial nucleoids with a commercially available DNA gel stain, acquisition of time lapse series of live labeled cells by super-resolution structured illumination microscopy (SR-SIM), and automatic tracking of nucleoid...
7.7K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

10.5K
High-resolution intravital imaging with enhanced contrast up to 120 µm depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 µm depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction...
10.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exposure to calcium stearyl lactylate induces hepatointestinal toxicity and gut microbiota dysbiosis in mice.

Current research in toxicology·2026
Same author

High-fidelity compressed high-speed imaging for resolving rapid micro-dynamics.

Optics express·2026
Same author

[Retracted] Novel triazole analogs of apigenin-7-methyl ether exhibit potent antitumor activity against ovarian carcinoma cells via the induction of mitochondrial-mediated apoptosis.

Experimental and therapeutic medicine·2026
Same author

Sappanone A exerted promising therapeutic effects in vitiligo through Wnt5a-mediated noncanonical Wnt signaling.

Journal of natural medicines·2026
Same author

Lensless quantitative microscopy based on multi-angle illumination and coded ptychographic phase retrieval.

Optics letters·2026
Same author

Evaluating the effectiveness of preventive training programs in reducing the incidence of knee injuries: a systematic review and meta-analysis.

Frontiers in public health·2026
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
14:11

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

Published on: May 4, 2014

16.3K

Multi-view object topography measurement with optical sectioning structured illumination microscopy.

Feifei Ren, Zhaojun Wang, Jia Qian

    Applied Optics
    |September 11, 2019
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel multi-view imaging method to overcome limitations in 3D surface topography measurement. The technique enhances optical sectioning structured illumination microscopy, enabling the detection of previously hidden surface details.

    More Related Videos

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.9K
    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    26.1K

    Related Experiment Videos

    Last Updated: Jan 19, 2026

    Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
    14:11

    Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

    Published on: May 4, 2014

    16.3K
    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    11.9K
    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
    11:15

    A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

    Published on: May 30, 2016

    26.1K

    Area of Science:

    • Optical microscopy
    • 3D surface metrology
    • Image processing

    Background:

    • Traditional 3D surface topography instruments face limitations with steep object slopes due to finite objective collection angles, leading to 'shadow problems'.
    • Existing methods struggle to capture details on surfaces with high local slopes, hindering comprehensive analysis.

    Purpose of the Study:

    • To develop a method that overcomes the 'shadow problem' in 3D surface topography measurement.
    • To enhance the capabilities of optical sectioning structured illumination microscopy for capturing details on challenging surfaces.

    Main Methods:

    • A multi-view image acquisition strategy was employed to capture object images from various angles.
    • A novel multi-view image fusion algorithm was developed to reconstruct a single, comprehensive 3D image.
    • The method extends the collection angle range of optical sectioning structured illumination microscopy.

    Main Results:

    • Previously invisible surface details on objects with slopes exceeding the objective's collection angle were successfully detected.
    • The reconstructed 3D images provided enhanced height map measurement and quantitative analysis capabilities.
    • The 'shadow problem' was effectively mitigated, allowing for more complete surface characterization.

    Conclusions:

    • The proposed multi-view imaging and fusion approach significantly improves 3D surface topography measurement, especially for complex surfaces.
    • This technique offers a valuable tool for detailed height map measurement and quantitative analysis in diverse scientific and industrial fields.
    • The method expands the applicability of optical sectioning microscopy for high-resolution surface characterization.