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Updated: Jan 19, 2026

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading
Published on: May 22, 2021
Robust edge-spread function construction methods to counter poor sample spacing uniformity in the slanted-edge method
Abstract:
The slanted-edge method describes an algorithm for measuring the spatial frequency response of digital imaging systems. The method can be applied to edges oriented at nearly any angle, but there are some angles that cause simplistic implementations of the algorithm to fail, or produce inaccurate measurements. These angle-dependent phenomena are demonstrated to stem from a lack of uniformity in supersample spacing in the edge-spread function. Two well-known slanted-edge implementation variants are adapted to minimize edge-orientation-dependent errors. These robust slanted-edge implementations are demonstrated to yield accurate measurements, regardless of edge orientation angle or moderate image noise.
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