Robust edge-spread function construction methods to counter poor sample spacing uniformity in the slanted-edge method

Related Concept Videos

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading10:54

Quantitative Analysis of Cell Edge Dynamics during Cell Spreading

In this protocol, we present the experimental procedures of a cell spreading assay that is based on live-cell microscopy. We provide an open-source computational tool for the unbiased segmentation of fluorescently labeled cells and quantitative analysis of lamellipodia dynamics during cell...
5.9K
Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy05:50

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

This protocol aims to measure the dynamic parameters (protrusions, retractions, ruffles) of protrusions at the edge of spreading...
2.8K
Identification of Pre-weanling 7-Day-Old Mice with Pinna Edge Biopsies04:06

Identification of Pre-weanling 7-Day-Old Mice with Pinna Edge Biopsies

The protocol presents a novel stepwise approach to producing a pinna-edge biopsy (PEB) in post-natal day (PND) 7 mice for the purpose of identification and simultaneous...
419
Fabrication of Uniform Nanoscale Cavities via Silicon Direct Wafer Bonding10:32

Fabrication of Uniform Nanoscale Cavities via Silicon Direct Wafer Bonding

A method for permanently bonding two silicon wafers so as to realize a uniform enclosure is described. This includes wafer preparation, cleaning, RT bonding, and annealing processes. The resulting bonded wafers (cells) have uniformity of enclosure ~1%1,2. The resulting geometry allows for measurements of confined liquids and...
10.2K
Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy10:25

Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

The full process from brain specimen preparation to serial sectioning imaging using the Knife-Edge Scanning Microscope, to data visualization and analysis is described. This technique is currently used to acquire mouse brain data, but it is applicable to other organs, other...
18.2K
Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

This protocol outlines the tape method on how to manually construct a tissue microarray using FFPE donor blocks of differing...
14.2K