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Related Experiment Video

Updated: Jan 19, 2026

High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue
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3D microphotonic probe for high resolution deep tissue imaging.

Mohammad Amin Tadayon, Shriddha Chaitanya, Kelly Marie Martyniuk

    Optics Express
    |September 13, 2019
    PubMed
    Summary

    Researchers developed a 100 µm ultra-compact optical probe for minimally invasive imaging. This microendoscopic tool achieves 1 µm resolution, overcoming current limitations in deep tissue imaging.

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    Area of Science:

    • Optical Engineering
    • Biomedical Imaging
    • Microfabrication

    Background:

    • Minimally invasive imaging requires ultra-compact optical components for microendoscopic tools and microscopes.
    • Current microendoscopic technologies face limitations in diameter and resolution due to manufacturing constraints.

    Purpose of the Study:

    • To demonstrate a platform for miniaturizing optical imaging systems for microendoscopic applications.
    • To achieve a resolution of 1 µm for deep tissue imaging.

    Main Methods:

    • Designed a probe using cascaded micro-lenses and waveguides (lensguide).
    • Fabricated the polymeric probe using photolithography and two-photon polymerization.
    • Achieved a probe size of 100 µm x 100 µm with a 60 µm field of view.

    Main Results:

    • Demonstrated a miniaturized optical imaging system with 1 µm resolution.
    • Successfully created an ultra-compact probe suitable for microendoscopic applications.
    • Enabled wide-field microscopy using the developed polymeric probe.

    Conclusions:

    • The developed lensguide platform offers a viable solution for creating ultra-compact microendoscopic imaging systems.
    • This technology overcomes current manufacturing restrictions, enabling higher resolution and smaller diameters.
    • The system holds significant potential for advancing minimally invasive diagnostic and surgical procedures.