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Rapid Amplification of cDNA Ends09:44

Rapid Amplification of cDNA Ends

Source: Pablo Sanchez Bosch2, Sean Corcoran2 and Katja Brückner1,2,3
1Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research
2Department of Cell and Tissue Biology, 
3Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA    
Rapid Amplification of cDNA Ends (RACE) is a technique that allows amplification of full-length cDNA from mRNA by extending to the 3’ or 5’ end, even without prior...
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Mutual Exclusivity: How Children Learn the Meanings of Words05:18

Mutual Exclusivity: How Children Learn the Meanings of Words

Source: Laboratories of Nicholaus Noles and Judith Danovitch—University of Louisville
Humans are different from other animals in many ways, but perhaps the most important differentiating factor is their ability to use language. Other animals can communicate and even understand and use language in limited ways, but trying to teach human language to a chimp or a dog takes a great deal of time and effort. In contrast, young humans acquire their native language easily, and they learn...
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Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification08:55

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

This protocol describes the isolation, culture, and calcification of rat-derived valve interstitial cells, a highly physiological in vitro model of calcific aortic valve disease (CAVD). Exploitation of this rat model facilitates CAVD research in exploring the cell and molecular mechanisms that underlie this complex pathological...
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Robust 3D DNA FISH Using Directly Labeled Probes12:16

Robust 3D DNA FISH Using Directly Labeled Probes

We describe a robust and versatile protocol for analyzing nuclear architecture by 3D DNA FISH using directly labeled fluorescent...
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Using the Endoscope for Endobronchial Ultrasound in the Esophagus04:35

Using the Endoscope for Endobronchial Ultrasound in the Esophagus

Transesophageal ultrasound (EUS-B) is a safe and feasible procedure using the echoendobronchoscope (EBUS) in esophagus and stomach. After identifying six anatomical landmarks, additional structures can be identified and biopsied, sparing subsequent diagnostic sessions. Thus, EUS-B is an ideal continuation of bronchoscopy and EBUS in diagnosing lung cancer and other...
1.0K
Numerical Cognition: More or Less05:59

Numerical Cognition: More or Less

Source: Laboratories of Nicholaus Noles and Judith Danovitch—University of Louisville
One of the goals of the modern education system is to teach children mathematical literacy. They are taught to add, subtract, multiply, and divide, and this base knowledge is used to support learning about geometry, algebra, calculus, physics, and statistics. School-aged children usually acquire these skills in formal educational settings, but the foundation of mathematical understanding is developed...
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