Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulating Schwann Cell Growth In Vitro Using the Nanosecond Pulsed Electric Field Technique02:15

Regulating Schwann Cell Growth In Vitro Using the Nanosecond Pulsed Electric Field Technique

345
Source: Han, J., et.al. Regulating Schwann Cell Growth by Nanosecond Pulsed Electric Field for Peripheral Nerve Regeneration In Vitro. J. Vis. Exp. (2024)This video demonstrates the application of the nanosecond pulsed electric field (nsPEF) technique to modulate Schwann cell (SC) growth. The rapid electric pulses induce transient nanopores in SC membranes, facilitating calcium influx that activates signaling pathways driving SC proliferation, dedifferentiation, and neurotrophic factor...
345
Regulating Schwann Cell Growth by Nanosecond Pulsed Electric Field for Peripheral Nerve Regeneration In Vitro09:24

Regulating Schwann Cell Growth by Nanosecond Pulsed Electric Field for Peripheral Nerve Regeneration In Vitro

1.4K
Here, we present a protocol for applying nanosecond pulse electric field (nsPEF) to stimulate Schwann cells in vitro. The synthesis and secretion ability of relevant factors and cell behavior changes validated the successful stimulation using nsPEF. The study gives a positive view of the peripheral nerve regeneration...
1.4K
AC Synchronous Machine Synchronization09:02

AC Synchronous Machine Synchronization

22.2K
Source: Ali Bazzi, Department of Electrical Engineering, University of Connecticut, Storrs, CT.
Three-phase wound-rotor synchronous generators are the main source of electrical power worldwide. They require a prime mover and an exciter in order to generate power. The prime mover can be a turbine spun by fluid (gas or liquid), thus the sources of the fluid can be water running off a dam through a long nozzle, steam from water evaporated using burned coal, etc. Most power plants including coal,...
22.2K
Bipolar Disorder01:30

Bipolar Disorder

640
Bipolar disorder is a chronic mental health condition marked by significant mood fluctuations, including episodes of mania and depression. Elevated energy levels, heightened mood or irritability, impulsive behavior, reduced sleep needs, rapid speech, racing thoughts, inflated self-esteem, and distractibility characterize mania. Individuals with bipolar disorder often alternate between depressive and manic states, with periods of emotional stability lasting an average of six months to a year.
640
All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics11:33

All-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics

10.2K
We demonstrate an all-electronic method to observe nanosecond-resolved charge dynamics of dopant atoms in silicon with a scanning tunneling...
10.2K
Pulse01:16

Pulse

2.0K
When the heart pumps blood out, arterial elastic fibers play a crucial role in sustaining a high-pressure gradient. They expand to accommodate the received blood and then recoil - a process known as the pulse that can be either manually palpated or electronically quantified. Despite a reduction in its effect with increased distance from the heart, elements of the pulse's systolic and diastolic components persist, observable even at the arteriole level.
The pulse serves as a clinical...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Digital droplet microfluidics integrating DNA walkers and CRISPR-Cas13a for simultaneous surface protein and miRNA profiling in single exosomes.

Biosensors & bioelectronics·2026
Same author

Discriminating Post-Transplant Rejection from Infection by Detecting TCR-CD3 Oligomerization on Extracellular Vesicles Using a Ratiometric Caliper Probe.

Journal of the American Chemical Society·2026
Same author

Establishment and Histopathological Characterization of a KYSE-30 Subcutaneous Xenograft Model of Esophageal Squamous Cell Carcinoma.

Cancers·2026
Same author

A CRISPR-Cas12a system integrated with metal-enhanced light-up aptamer-fluorophore nanoreporter for ultrasensitive detection of prostate-specific antigen.

Biosensors & bioelectronics·2026
Same author

Generalized additive model integrating multi-source data for short-term influenza forecasting in Shenzhen, China (2023-2025).

Frontiers in public health·2026
Same author

The impact of El Niño-Southern Oscillation on the incidence of infectious diarrhea in China: insights from a 15-year national surveillance analysis.

Frontiers in public health·2026

Related Experiment Video

Updated: Jan 19, 2026

Regulating Schwann Cell Growth In Vitro Using the Nanosecond Pulsed Electric Field Technique
02:15

Regulating Schwann Cell Growth In Vitro Using the Nanosecond Pulsed Electric Field Technique

Published on: June 17, 2025

345

Selective distant electrostimulation by synchronized bipolar nanosecond pulses.

Elena C Gianulis1, Maura Casciola1, Carol Zhou1

  • 1Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, 23508, USA.

Scientific Reports
|September 13, 2019
PubMed
Summary
This summary is machine-generated.

Cancellation of bipolar cancellation (CANCAN-ES) uses synchronized nanosecond electric pulses (nsEP) to create a remote unipolar pulse. This novel electrostimulation method enhances cell permeability, overcoming limitations of traditional bipolar nsEP.

More Related Videos

Author Spotlight: Innovative Use of nsPEF to Boost Peripheral Nerve Regeneration
09:24

Author Spotlight: Innovative Use of nsPEF to Boost Peripheral Nerve Regeneration

Published on: May 3, 2024

1.4K
AC Synchronous Machine; Prime-Mover Initialization
09:02

AC Synchronous Machine; Prime-Mover Initialization

Published on: April 30, 2023

22.2K

Related Experiment Videos

Last Updated: Jan 19, 2026

Regulating Schwann Cell Growth In Vitro Using the Nanosecond Pulsed Electric Field Technique
02:15

Regulating Schwann Cell Growth In Vitro Using the Nanosecond Pulsed Electric Field Technique

Published on: June 17, 2025

345
Author Spotlight: Innovative Use of nsPEF to Boost Peripheral Nerve Regeneration
09:24

Author Spotlight: Innovative Use of nsPEF to Boost Peripheral Nerve Regeneration

Published on: May 3, 2024

1.4K
AC Synchronous Machine; Prime-Mover Initialization
09:02

AC Synchronous Machine; Prime-Mover Initialization

Published on: April 30, 2023

22.2K

Area of Science:

  • Biophysics
  • Cell Biology
  • Biomedical Engineering

Background:

  • Electrostimulation (ES) with nanosecond electric pulses (nsEP) exhibits reduced biostimulation efficiency with bipolar pulses due to polarity reversal.
  • Bipolar cancellation inherently limits the effectiveness of nsEP for therapeutic applications.

Purpose of the Study:

  • To introduce and validate the cancellation of bipolar cancellation electrostimulation (CANCAN-ES) paradigm.
  • To achieve remote biostimulation by overcoming the limitations of bipolar cancellation in nsEP.

Main Methods:

  • Utilizing two independent generators to deliver phase-shifted bipolar nsEP.
  • Synchronizing nsEP to create a remote unipolar pulse through constructive interference.
  • Quantifying cell membrane permeabilization via YO-PRO-1 dye uptake in CHO-K1 cells.

Main Results:

  • CANCAN-ES successfully generated a remote unipolar pulse, confirmed by enhanced YO-PRO-1 dye uptake.
  • A ~3-fold increase in dye uptake was observed in the electrode gap center, matching the predicted unipolar pulse location.
  • The enhancement in cell permeability directly correlated with the degree of bipolar cancellation, validating the CANCAN-ES principle.

Conclusions:

  • CANCAN-ES effectively restores stimulation efficiency by canceling bipolar cancellation, enabling remote biostimulation.
  • This technique holds promise for non-invasive deep tissue stimulation.
  • CANCAN-ES can be integrated with other remote stimulation methods for improved targeting precision.