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Related Concept Videos

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer09:52

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

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We describe a dramatically improved method for mouse cloning using trichostatin A, vitamin C, and deionized bovine serum albumin. We show a simplified, reproducible protocol that supports efficient development of cloned embryos. Hence, this method could become a standardized procedure for mouse...
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This movie and the protocol are intended to help learning nuclear...
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Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
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Molecular cloning is a set of methods, which are used to insert recombinant DNA into a vector - a carrier of DNA molecules that will replicate recombinant DNA fragments in host organisms. The DNA fragment, which may be a gene, can be isolated from a prokaryotic or eukaryotic specimen. Following isolation of the fragment of interest, or insert, both the vector and insert must be cut with restriction enzymes and purified. The purified pieces are joined together though a technique called...
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Protons and neutrons, collectively called nucleons, are packed together tightly in a nucleus. With a radius of about 10−15 meters, a nucleus is quite small compared to the radius of the entire atom, which is about 10−10 meters. Nuclei are extremely dense compared to bulk matter, averaging 1.8 × 1014 grams per cubic centimeter. If the earth’s density were equal to the average nuclear density, the earth’s radius would be only about 200 meters.
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The process of converting very light nuclei into heavier nuclei is also accompanied by the conversion of mass into large amounts of energy, a process called fusion. The principal source of energy in the sun is a net fusion reaction in which four hydrogen nuclei fuse and ultimately produce one helium nucleus and two positrons.
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Related Experiment Video

Updated: Jan 19, 2026

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
09:52

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Published on: April 26, 2018

13.1K

Nuclear Transfer and Cloning.

Ling Liu1

  • 1IVF Canada Fertility Center, Toronto, ON, Canada. lingliu@hotmail.com.

Methods in Molecular Biology (Clifton, N.J.)
|September 13, 2019
PubMed
Summary

Nuclear transfer (NT) cloning enables gene reprogramming studies and live offspring creation. This summary updates techniques for generating mice via NT cloning, building on earlier protocols.

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Genetics

Background:

  • Nuclear transfer (NT) is a key technique for gene reprogramming and cloning.
  • It involves enucleated oocytes and donor cell nuclei for embryo development.
  • Previous protocols for mouse NT were established in 1998 and 2000.

Purpose of the Study:

  • To provide a comprehensive update on mouse NT cloning techniques.
  • To summarize the latest publications on NT protocols for generating mice.
  • To highlight the experimental utility of NT in research.

Main Methods:

  • Enucleation of oocytes.
  • Injection of donor cell nuclei into enucleated oocytes.
  • Activation of reconstructed oocytes and subsequent embryonic development.
Keywords:
CloningEmbryo transferMouseNuclear transferOocyte activationOocytesSomatic cells

More Related Videos

Nuclear Transfer into Mouse Oocytes
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Nuclear Transfer into Mouse Oocytes

Published on: November 30, 2006

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Reproductive Cloning and Somatic Cell Nuclear Transfer
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Related Experiment Videos

Last Updated: Jan 19, 2026

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
09:52

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Published on: April 26, 2018

13.1K
Nuclear Transfer into Mouse Oocytes
14:17

Nuclear Transfer into Mouse Oocytes

Published on: November 30, 2006

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Reproductive Cloning and Somatic Cell Nuclear Transfer
01:27

Reproductive Cloning and Somatic Cell Nuclear Transfer

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Main Results:

  • Reprogramming of donor cell nuclei within the oocyte cytoplasm.
  • Development of reconstructed oocytes into embryos with donor cell genomes.
  • Generation of cloned mice from reconstructed embryos.

Conclusions:

  • Nuclear transfer cloning is a powerful tool for biological research.
  • Updated protocols enhance the efficiency and application of mouse NT.
  • NT cloning facilitates studies in gene reprogramming and developmental biology.