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Updated: Jan 19, 2026

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
Published on: April 23, 2014
Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells Using Sendai Virus
Julia M Draper1, Jay L Vivian2,3
1Transgenic and Gene-Targeting Institutional Facility, University of Kansas Medical Center, Kansas City, KS, USA.
Abstract:
Induced pluripotent stem (iPS) cells are important tools for studying differentiation and for use in patient-specific disease modeling. We present a detailed method for the reprogramming of primary human fibroblasts to induced pluripotent stem cells using Sendai virus. These procedures allow for the efficient generation of multiple high-quality feeder-independent iPS cell lines for a given human fibroblast line. The iPS cell lines generated by this protocol can be used in a variety of differentiation and gene expression studies, as well as in genetic manipulations.
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