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Related Concept Videos

Tree Core Analysis with X-ray Computed Tomography06:56

Tree Core Analysis with X-ray Computed Tomography

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Here we show how to process tree cores with an X-ray computed tomography toolchain. Except for chemical extraction for some purposes, no further physical lab treatment is needed. The toolchain can be used for biomass estimations, for obtaining MXD/tree-ring width data as well as for obtaining quantitative wood anatomy data.
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Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation07:54

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Here, we present a protocol on how to determine the quantity and distribution of metals in a sample using synchrotron X-ray fluorescence. We focus on adherent cells, and describe the chemical fixation method to prepare this sample. We then describe how to mount and image the sample using synchrotron X-rays.
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Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating10:39

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The measurement protocol and data analysis procedure are given for obtaining transverse coherence of a synchrotron radiation X-ray source along four directions simultaneously using a single 2-D checkerboard phase grating. This simple technique can be applied for complete transverse coherence characterization of X-ray sources and X-ray...
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X-ray Fluorescence (XRF)07:45

X-ray Fluorescence (XRF)

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Source: Laboratory of Dr. Lydia Finney — Argonne National Laboratory
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3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography07:01

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A protocol for 3D visualization of microscopic tissue structures by using an X-ray specific staining method designed for X-ray computed tomography is...
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Extracting Metrics for Three-dimensional Root Systems: Volume and Surface Analysis from In-soil X-ray Computed Tomography Data09:37

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A methodology for obtaining visual and quantitative root structure information from X-ray computed tomography data acquired in-soil is...
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Related Experiment Video

Updated: Jan 19, 2026

Author Spotlight: Advancements in X-ray CT Tool Chain for Tree Core Analysis
06:56

Author Spotlight: Advancements in X-ray CT Tool Chain for Tree Core Analysis

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Reduced acquisition time for L-shell x-ray fluorescence computed tomography using polycapillary x-ray optics.

Don Vernekohl1, Moiz Ahmad2, Xianjin Dai1

  • 1Department of Radiation Oncology, Stanford University, 450 Serra Mall, Stanford, CA, 94305, USA.

Medical Physics
|September 13, 2019
PubMed
Summary
This summary is machine-generated.

X-ray fluorescence computed tomography (XFCT) imaging time was significantly reduced using polycapillary optics. This advancement allows for faster detection of contrast agents in preclinical imaging.

Keywords:
XFCThigh sensitvitymolecular imagingpolycapillarsmall animalx-ray

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Area of Science:

  • Medical Imaging
  • Biomedical Engineering
  • Physics

Background:

  • X-ray fluorescence computed tomography (XFCT) is an advanced molecular imaging technique.
  • It offers high atomic number contrast and deep tissue penetration, surpassing other modalities.
  • Current limitations include lengthy acquisition times, hindering clinical translation.

Purpose of the Study:

  • To demonstrate reduced acquisition times for L-shell XFCT imaging.
  • To utilize x-ray focusing polycapillary optics for enhanced performance.
  • To improve XFCT's feasibility for preclinical and clinical applications.

Main Methods:

  • A prototype XFCT system with a custom polycapillary focusing optic was developed.
  • The optic increased X-ray fluence rate by tenfold compared to standard collimation.
  • Phantoms with varying gold concentrations were imaged using a silicon drift detector (SDD).

Main Results:

  • XFCT acquisition time was reduced from 17 hours to under 1 hour.
  • The polycapillary optic improved sensitivity by increasing fluence rate and reducing scatter.
  • Gold concentrations as low as 60 μg/mL were visualized, with 300 μg/mL detected in a mouse phantom within 66 minutes.

Conclusions:

  • High fluence rate from polycapillary x-ray sources significantly reduces signal integration time.
  • L-shell XFCT can detect subtle contrast agent amounts within biologically relevant timeframes.
  • Polycapillary x-ray source technology is suitable for preclinical L-shell XFCT; integrating more SDDs can further enhance sensitivity and reduce dose.