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Updated: Jan 19, 2026
Extraction and Analysis of Taiwanese Green Propolis
Published on: January 7, 2019
Simple and Efficient Green Extraction of Steviol Glycosides from Stevia rebaudiana Leaves
Verónica López-Carbón1,2, Ana Sayago3,4, Raúl González-Domínguez5,6
1Department of Chemistry, Faculty of Experimental Sciences, University of Huelva, 21007 Huelva, Spain. veronica.carbon@alu.uhu.es.
Abstract:
The food industry has currently shown great interest in alternative sweeteners to sugars with the aim of producing healthier products. In light of this, steviol glycosides are natural low-caloric sweeteners present in Stevia rebaudiana, which have additionally been described as bioactive components with potential therapeutic properties. In this work, a green method for the extraction of steviol glycosides from stevia leaves was optimized by applying a factorial screening design of five variables (time, temperature, agitation, grinding, and sample-solvent ratio) and the subsequent response surface design of Box-Behnken. The optimized extraction method allows for the recovery of stevia sweeteners in a simple and efficient manner by using tap water as the extractant, without the application of an auxiliary energy source to reduce costs, thus representing an interesting strategy for their industrial-scale production.
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