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A persistent luminescence-based label-free probe for the ultrasensitive detection of hemoglobin in human serum

Yihui Liu1, Yuhui Wang2, Kai Jiang2

  • 1School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, 315211, PR China; Key Laboratory of Additive Manufacturing Materials of Zhejiang Province, Ningbo Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China.

Talanta
|September 14, 2019
PubMed

Hemoglobin (Hb) plays an important role in oxygen carriage for mammals, which is also a typical biomarker for certain diseases. Although numerous methods had been developed for the detection of Hb in red blood cells, analytical technology for the monitoring of low-abundance Hb in serum or plasma is still a challenge. Herein, persistent luminescence nanoparticles (PLNPs) with strong near-infrared (NIR) emission character behaving as a label-free probe for the highly sensitive and selective detection of Hb were developed. Further studies revealed that the sensing mechanism should be attributed to the Hb-induced dynamic quenching process. Moreover, the nanoprobe showed high selectivity to Hb against the common existing substances in human serum and a linear response to Hb ranging from 1 to 50 nM with an extremely high limit of detection (LOD) of 0.13 nM. Finally, applicability of the proposed probe for the detection of Hb in human serum samples was validated.

Keywords:
Hemoglobin detectionHuman serum sampleLabel-free probeNear-infrared emissionPersistent luminescence

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