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In Vivo Imaging Uncovers the Migratory Behavior of Leukocytes within the Joints10:10

In Vivo Imaging Uncovers the Migratory Behavior of Leukocytes within the Joints

In vivo imaging of the joints is a valuable technique for uncovering the migratory behavior of leukocytes and molecular dynamics within the inflamed joints. Here, we present a detailed protocol for in vivo imaging of the joints using a murine model of type II collagen-induced arthritis...
558
Minimal Erythema Dose (MED) Testing06:24

Minimal Erythema Dose (MED) Testing

This article describes how to conduct minimal erythema dose (MED) testing in order to determine the lowest dose of ultraviolet radiation that will cause erythema (burning) when administered to an...
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Wound Assay to Evaluate the Migratory Capacity of Murine Myoblasts in Co-Culture05:00

Wound Assay to Evaluate the Migratory Capacity of Murine Myoblasts in Co-Culture

This video describes a non-contact co-culture wound healing assay to evaluate the migratory capacity of murine myoblasts. This helps to understand signal-sending and signal-receiving components of the paracrine non-canonical Wnt signaling interactions in...
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Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response06:31

Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response

Minimal erythema dose (MED) testing is used to establish dosage schedules for ultraviolet radiation phototherapy. It can assess individual variation in inflammatory response but lacks methodology for achieving reproducible results. Here, we present a precision implementation of MED and demonstrate its ability to capture individual variation in inflammatory...
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Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures06:34

Migratory Behavior of Cells Generated in Ganglionic Eminence Cultures

Time lapse imaging of 3D tissue culture allows studying migratory behavior of individual cells originating from ganglionic eminence in reaction to fractionated protein extract from cerebral...
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Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology10:07

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust...
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