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Related Experiment Video

Updated: Jan 19, 2026

Isolation of Small Noncoding RNAs from Human Serum
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Pollen-derived RNAs Are Found in the Human Circulation.

Milka Koupenova1, Eric Mick2, Heather A Corkrey1

  • 1Department of Medicine, Division of Cardiovascular Medicine, University of Massachusetts Medical School, 368 Plantation St., AS7-1051, Worcester, MA 01605, USA.

Iscience
|September 14, 2019
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Summary

Non-human microRNAs (miRNAs) from pine pollen can enter human circulation through the lungs. These plant miRNAs were detected in people and linked to hay fever, not major diseases.

Keywords:
Biological SciencesMolecular BiologyOmicsTranscriptomics

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Area of Science:

  • Molecular Biology
  • Immunology
  • Environmental Health

Background:

  • The presence and origin of nonhuman RNAs, specifically microRNAs (miRNAs), in human circulation remain largely uncharacterized.
  • Understanding the potential transfer of food- or environment-derived miRNAs into the human bloodstream is crucial for health and disease research.

Purpose of the Study:

  • To investigate the presence of nonhuman miRNAs in human plasma.
  • To determine the origin and mechanism of transfer of plant-derived miRNAs into circulation.
  • To explore associations between detected plant miRNAs and human health conditions.

Main Methods:

  • RNA sequencing was employed on plasma samples from a large population study.
  • Quantitative real-time PCR (RT-qPCR) was used to validate specific pine-pollen miRNAs.
  • In vivo and in vitro models were utilized to elucidate the transfer mechanism via pulmonary pathways.
  • Platelet-pulmonary vascular cell interactions and platelet pollen-DNA uptake were examined.

Main Results:

  • Nonhuman miRNAs were detected in human plasma.
  • A subset of pine-pollen miRNAs was validated in 2,776 individuals.
  • The presence of these pine-pollen miRNAs correlated with hay fever symptoms.
  • No association was found with overt cardiovascular or pulmonary diseases.
  • Mechanistic studies revealed pulmonary transfer mediated by platelets and vascular cells.

Conclusions:

  • Pollen-derived plant miRNAs can be horizontally transferred into human circulation through the pulmonary system.
  • Platelet-mediated interactions play a key role in the pulmonary transfer of plant miRNAs.
  • While mechanistic plausibility is established, the current observational data do not link these plant miRNAs to major diseases or risk factors.