Does lower brain-derived neurotrophic factor in adolescent waterpipe smokers suggest a negative effect on the...

We present a protocol to generate a human brain organoid with resident microglia by incorporating Induced pluripotent stem cell (iPSC)-derived hematopoietic progenitor cells (HPCs) into organoid...

This article describes a method by which one can mimic in vivo development of the Drosophila mushroom body in an ex vivo culture...

During adolescence, individuals experience significant cognitive development that enhances their understanding of others' emotions and thoughts, known as cognitive empathy. This period is marked by an increased ability to adapt to others' perspectives and a more nuanced understanding of others' mental states, a skill that is foundational for social problem-solving and conflict avoidance. The development of cognitive empathy relies heavily on the theory of mind — the...

Single-cell electroporation (SCE) is a specialized technique allowing delivery of DNA or other macromolecules into individual cells within intact tissue, including in vivo preparations. Here we detail the procedure for SCE of a fluorescent dye or plasmid DNA into neurons within the intact brain of the Xenopus laevis...

This video showcases creating a blood-brain barrier chip using iPSC-derived precursor cells. It involves seeding neural progenitor cells and brain microvascular endothelial cells in separate channels with a porous membrane. By flowing differentiation media, the endothelial cells form tight junctions resembling blood vessels, and the neural cells mature into various brain cell types, establishing a functional blood-brain barrier on the...

Primary, human fetal brain-derived, multipotential progenitor cells proliferate in vitro while maintaining the capacity to differentiate into neurons and astrocytes. This work shows that neural progenitors can be induced to differentiate through stages of the oligodendrocytic lineage by conditioning with select growth...