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Updated: Jan 19, 2026
Preparation of Binary and Ternary Deep Eutectic Systems
Published on: October 31, 2019
Selectivity of deproteinization and demineralization using natural deep eutectic solvents for production of insect
Pengfei Zhou1, Jingbo Li2, Tao Yan3
1Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, 510610, China.
Abstract:
Selectivity of deproteinization and demineralization using natural deep eutectic solvents for preparation of insect chitin (Hermetia illucens) was investigated. The relationships between the pH and pKa values of NADESs and demineralization, deproteinization and crystallinity indexes of the obtained chitin were discussed, respectively. It was found that the acidic NADESs consist of basic hydrogen bond acceptor (HBA) and acidic hydrogen bond donor (HBD), or the alkali NADESs composed of acid HBA and alkali HBD were better for chitin products. After reused for three times, demineralization and deproteinization abilities of NADESs were not significantly decreased. Mechanistic analysis indicated that H+ released from HBA or HBD was the main reason for demineralization, whereas intermolecular and intramolecular hydrogen bond in NADESs facilitates the removal of protein. Overall, this report provides a preliminary reference for design of the sustainable NADESs for preparation of chitin from natural resources.
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