Related Experiment Video

Updated: Jan 19, 2026

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
09:00

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

Published on: August 4, 2009

28.8K

Highly sensitive fluorescence imaging of cancer with avidin-protease probe conjugate

Kyoko Yamamoto1, Mako Kamiya2, Yasuteru Urano3

  • 1Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Bioorganic & Medicinal Chemistry Letters
|September 16, 2019
PubMed

Keywords:
AvidinCancer imagingClick chemistryFluorescence probesLysosomal protease

More Related Videos

An Avidin-Biotin Conjugation Technique for Presenting Target Antigens on Mycobacterium bovis BCG03:12

An Avidin-Biotin Conjugation Technique for Presenting Target Antigens on Mycobacterium bovis BCG

451
Detection of Protease Activity by Fluorescent Peptide Zymography09:56

Detection of Protease Activity by Fluorescent Peptide Zymography

Published on: January 20, 2019

13.5K

Related Concept Videos

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity09:00

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein...
28.8K
An Avidin-Biotin Conjugation Technique for Presenting Target Antigens on Mycobacterium bovis BCG03:12

An Avidin-Biotin Conjugation Technique for Presenting Target Antigens on Mycobacterium bovis BCG

The video demonstrates a technique for loading antigens on Mycobacterium bovis BCG to improve its immunogenic properties. The method uses the avidin-biotin system to coat the bacterial surface with exogenous...
451
Detection of Protease Activity by Fluorescent Peptide Zymography09:56

Detection of Protease Activity by Fluorescent Peptide Zymography

Here, we present a detailed protocol for a modified zymographic technique in which fluorescent peptides are used as the degradable substrate in place of native proteins. Electrophoresis of biological samples in fluorescent peptide zymograms enables detection of a wider range of proteases than previous zymographic techniques.
13.5K
A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

The present protocol describes a detailed, real-time NIR-II fluorescence imaging operation of a mouse using a NIR-II optics imaging...
2.3K
Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)08:14

Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)

Here, we present a protocol to determine the orientation and topology of integral membrane proteins in living cells. This simple protocol relies on selective protease sensitivity of chimeras between the protein of interest and GFP.
18.3K
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar...
9.5K