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Multilocus Sequence Typing of Staphylococcus aureus.

Ying Liu1, Yinduo Ji2

  • 1Shanghai Vocational College of Agriculture and Forestry, Shanghai, China.

Methods in Molecular Biology (Clifton, N.J.)
|September 17, 2019
PubMed
Summary
This summary is machine-generated.

Multilocus sequence typing (MLST) differentiates bacterial strains like Staphylococcus aureus. This method identified three common sequence types (ST5, ST30, ST8) in pediatric cystic fibrosis patients, aiding infectious disease tracking.

Keywords:
GenotypingMLSTMRSAStaphylococcus aureus

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Area of Science:

  • Microbiology
  • Epidemiology
  • Genetics

Background:

  • Multilocus sequence typing (MLST) is a robust method for bacterial strain differentiation and tracking.
  • MLST analyzes internal housekeeping gene sequences for molecular epidemiological characterization.
  • Staphylococcus aureus (S. aureus) is a significant pathogen where MLST is applicable.

Purpose of the Study:

  • To detail the procedure for Multilocus Sequence Typing (MLST) for S. aureus genotyping.
  • To present findings from previous MLST analysis of S. aureus isolates from pediatric cystic fibrosis patients.

Main Methods:

  • Utilizing MLST based on the sequences of seven conserved housekeeping genes.
  • Applying the MLST protocol to S. aureus isolates.

Main Results:

  • Previous studies identified three prevalent sequence types (STs) in S. aureus isolates from pediatric cystic fibrosis patients: ST5, ST30, and ST8.
  • The described procedure facilitates accurate S. aureus genotyping.

Conclusions:

  • MLST is a valuable tool for the molecular epidemiological surveillance of S. aureus.
  • Understanding prevalent S. aureus genotypes in specific populations, like cystic fibrosis patients, is crucial for disease control.