Related Experiment Video
Updated: Jan 19, 2026

Associated Chromosome Trap for Identifying Long-range DNA Interactions
Published on: April 23, 2011
Genetic Manipulations of Staphylococcal Chromosomal DNA
Crystal M Austin1, Jeffrey L Bose2
1Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, USA.
Abstract:
Performing genetic manipulation is often key to understanding bacterial gene function. In this chapter, we present the method of allelic exchange using temperature-sensitive plasmids to generate mutations in Staphylococcus, including single-nucleotide mutations, insertions, and gene deletions. In addition, this chapter summarizes other key genetic technologies used for the manipulation of S. aureus, including the CRISPR/Cas9 system and complementation.
Related Concept Videos
14:49Associated Chromosome Trap for Identifying Long-range DNA Interactions
DNA as a Genetic Template
DNA as a Genetic Template
14:54Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
Polytene Chromosomes
Chromosome Structure
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...

