Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

2.0K
The protocol submitted here explains the complete in silico pipeline needed to predict and functionally characterize circRNAs from RNA-sequencing transcriptome data studying host-pathogen...
2.0K
Preparation of Streptococcus mitis Culture for Host-Pathogen Interaction Studies02:14

Preparation of Streptococcus mitis Culture for Host-Pathogen Interaction Studies

218
Source: Naji, A., et al. Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans. J. Vis. Exp. (2019).This video demonstrates the method for culturing Streptococcus mitis under microaerophilic conditions using Todd-Hewitt Yeast Extract, or THY agar medium, to prepare bacterial cultures for host-pathogen interaction...
218
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

4.7K
The protocol presented here describes a complete pipeline to analyze RNA-sequencing transcriptome data from raw reads to functional analysis, including quality control and preprocessing steps to advanced statistical analytical...
4.7K
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions05:37

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

7.4K
Here, we present a protocol to profile the interplay between host and pathogen during infection by mass spectrometry-based proteomics. This protocol uses label-free quantification to measure changes in protein abundance of both host (e.g., macrophages) and pathogen (e.g., Cryptococcus neoformans) in a single...
7.4K
Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions07:35

Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions

7.6K
Here, the protocol describes how to perform double labeling immunofluorescence using primary antibodies raised in the same species to study host-pathogen interactions. Also, it can include the third antibody from a different host in this protocol. This approach can be made in any cell type and...
7.6K
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

11.6K
This article focuses on the identification of high-confident interaction datasets between host and pathogen proteins using a combination of two orthogonal methods: yeast two-hybrid followed by a high-throughput interaction assay in mammalian cells called...
11.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

D-alanine aminotransferase (Dat) promotes <i>Staphylococcus aureus</i> colonization fitness on human nasal respiratory epithelium.

bioRxiv : the preprint server for biology·2026
Same author

<i>Staphylococcus aureus</i> inhibits the NLRP3 inflammasome in macrophages to varying degrees during early and late stages of infection.

Infection and immunity·2026
Same author

Osteomyelitis.

Nature reviews. Disease primers·2026
Same author

RANKL inhibits macrophage proinflammatory Toll-like receptor 2 and 4 signaling and impairs killing of intracellular bacteria.

ImmunoHorizons·2026
Same author

Erratum for Petronglo et al., "Context-Dependent Roles for Toll-Like Receptors 2 and 9 in the Pathogenesis of <i>Staphylococcus aureus</i> Osteomyelitis".

Infection and immunity·2026
Same author

Revealing community dynamics in polymicrobial infections through a quantitative framework.

ISME communications·2026

Related Experiment Video

Updated: Jan 19, 2026

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

2.0K

Identification of Virulence Determinants During Host-Pathogen Interaction Using Tn-Seq Technology.

Christopher T Peek1,2, Carolyn B Ibberson3,4, James E Cassat5,6,7,8

  • 1Vanderbilt Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 17, 2019
PubMed
Summary

Transposon sequencing (Tn-seq) identifies essential Staphylococcus aureus genes during infection. This genetic tool helps understand bacterial pathogenesis in specific host environments like osteomyelitis.

Keywords:
In vivoInvasive infectionPathogenesisS. aureusTn-seq osteomyelitisTransposon sequencing

More Related Videos

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.7K
Preparation of Streptococcus mitis Culture for Host-Pathogen Interaction Studies
02:14

Preparation of Streptococcus mitis Culture for Host-Pathogen Interaction Studies

Published on: October 30, 2025

218

Related Experiment Videos

Last Updated: Jan 19, 2026

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
10:27

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

2.0K
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

4.7K
Preparation of Streptococcus mitis Culture for Host-Pathogen Interaction Studies
02:14

Preparation of Streptococcus mitis Culture for Host-Pathogen Interaction Studies

Published on: October 30, 2025

218

Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • Transposon sequencing (Tn-seq) is a powerful genetic tool for identifying essential genes.
  • Staphylococcus aureus Tn-seq libraries have been generated for numerous strains.
  • Computational tools and Tn-seq are increasingly used in animal infection models.

Purpose of the Study:

  • To describe the application of Tn-seq in a murine model of osteomyelitis.
  • To elucidate mechanisms of bacterial pathogenesis using Tn-seq.
  • To identify essential Staphylococcus aureus genes in a specific host environment.

Main Methods:

  • Utilizing transposon sequencing (Tn-seq) technology.
  • Generating transposon libraries for Staphylococcus aureus.
  • Deploying Tn-seq in a murine model of osteomyelitis.

Main Results:

  • Tn-seq enables the detection of essential genes in a given environment.
  • Application of Tn-seq in animal models identifies essential S. aureus genes.
  • This technology is valuable for understanding bacterial pathogenesis.

Conclusions:

  • Tn-seq is a powerful tool for genetic analysis in Staphylococcus aureus.
  • The study provides a paradigm for using Tn-seq in osteomyelitis models.
  • Tn-seq aids in elucidating bacterial pathogenesis mechanisms within host environments.