Related Experiment Video
Updated: Jan 19, 2026

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
Published on: April 25, 2018
Nitrobenzyl-based fluorescent photocages for spatial and temporal control of signalling lipids in cells
Pankaj Gaur1, Oleksandr A Kucherak1, Yulia G Ermakova2
1Laboratory of Chemical Biology, The Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo namesti 2, 16610 Prague 6, Czech Republic. yushchenko@uochb.cas.cz.
Abstract:
Here we present a set of fluorescent cages prepared by tethering fluorescent dyes to a photolabile group. The developed molecules enable caging of signalling lipids, their delivery to specific cellular membranes, with further imaging, quantification, and controlled photorelease of active lipids in living cells.
Related Concept Videos
07:48Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
10:12Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
06:09Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells
09:39Spatial Temporal Analysis of Fieldwise Flow in Microvasculature
11:52Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
09:34Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

