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Updated: Jan 19, 2026

A Magnetic Nanoparticle-Based Immunoassay Using a Microfluidic Device
pH-responsive lignin-based magnetic nanoparticles for recovery of cellulase
Rui-Jing Dong1, Da-Feng Zheng1, Dong-Jie Yang1
1Guangdong Engineering Research Center for Green Fine Chemicals, College of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
Abstract:
Enzymatic hydrolysis of lignocellulose to produce bioethanol by cellulase is an important method to alleviate the energy crisis. In this paper, in order to overcome the shortcomings of low efficiency, high cost and easy deactivation of cellulase in the process of bio-refinery, pH-responsive lignin-based magnetic nanoparticles (Fe3O4/LSQA) were synthesized to immobilize and recover cellulase. It was shown that a high immobilization ratio of 55.52% for cellulase was obtained. Meanwhile, the desorption ratio was 68.27% by adjusting the pH of the system. After five reusing cycles, the desorbed cellulase retained 31.79% of the relative activity due to the pH responsiveness of Fe3O4/LSQA. These results not only provide a new idea for the recycling of cellulase, but also broaden the application of industrial lignin and increase the extra value.
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