pH-responsive lignin-based magnetic nanoparticles for recovery of cellulase

Rui-Jing Dong1, Da-Feng Zheng1, Dong-Jie Yang1

  • 1Guangdong Engineering Research Center for Green Fine Chemicals, College of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.

Bioresource Technology
|September 17, 2019
PubMed

Related Concept Videos

A Magnetic Nanoparticle-Based Immunoassay Using a Microfluidic Device04:16

A Magnetic Nanoparticle-Based Immunoassay Using a Microfluidic Device

This video demonstrates a microfluidic system for a magnetic nanoparticle-based immunoassay. The detection system utilizes a microfluidic device incorporating a magnetic trap within its channels. Pre-formed immunocomplexes, comprised of antigen-coated magnetic nanoparticles bound to primary and peroxidase-conjugated secondary antibodies, are introduced into the channels. The immunocomplexes become captured in the porous magnetic trap upon applying an external magnetic field. Using a fluorogenic...
702
Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis14:43

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

A double stranded RNA interference (dsRNAi) technique is employed to down-regulate the maize cinnamoyl coenzyme A reductase (ZmCCR1) gene to lower plant lignin content. Lignin down-regulation from the cell wall is visualized by microscopic analyses and quantified by the Klason method. Compositional changes in hemicellulose and crystalline cellulose are...
13.9K
Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles09:28

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Antigen-specific T cells are difficult to characterize or utilize in therapies due to their extremely low frequency. Herein, we provide a protocol to develop a magnetic particle which can bind to antigen-specific T cells to enrich these cells and then to expand them several hundred-fold for both characterization and therapy.
12.0K
Magnetic-, Acoustic-, and Optical-Triple-Responsive Microbubbles for Magnetic Hyperthermia and Pothotothermal Combination Cancer Therapy09:01

Magnetic-, Acoustic-, and Optical-Triple-Responsive Microbubbles for Magnetic Hyperthermia and Pothotothermal Combination Cancer Therapy

Presented here is a protocol for the fabrication of iron oxide nanoparticle-shelled microbubbles (NSMs) through self-assembly, synergizing magnetic, acoustic, and optical responsiveness in one nanotherapeutic platform for magnetic hyperthermia and photothermal combination cancer...
3.5K
In Vitro and In Vivo Delivery of Magnetic Nanoparticle Hyperthermia Using a Custom-Built Delivery System06:45

In Vitro and In Vivo Delivery of Magnetic Nanoparticle Hyperthermia Using a Custom-Built Delivery System

This protocol presents techniques and methodology necessary for the accurate delivery of magnetic nanoparticle hyperthermia using a sophisticated delivery and monitoring...
4.7K
A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

This protocol describes a high throughput screen for cellulolytic activity from a metagenomic library expressed in Escherichia coli. The screen is solution based and highly automated, and uses one-pot chemistry in 384 well microplates with the final readout as an absorbance...
16.5K