Related Concept Videos

Generating an Ex Vivo Ovine Wounded Skin Model Infected with a Bacterial Pathogen02:13

Generating an Ex Vivo Ovine Wounded Skin Model Infected with a Bacterial Pathogen

The video demonstrates the generation of an ex vivo ovine skin model infected with Staphylococcus aureus. This entails treating a lamb's forehead skin biopsy section with antibiotics, establishing a wound bed, positioning it on a permeable membrane in a multiwell plate with antibiotic-free medium, and establishing bacterial infection in the skin...
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A Murine Skin Wound Infection Model to Study the Immune Response against a Bacterial Pathogen04:00

A Murine Skin Wound Infection Model to Study the Immune Response against a Bacterial Pathogen

This video demonstrates a technique to generate a murine model of skin wound infection. Upon infecting mice — engineered to express a fluorescent protein in neutrophils — with a genetically modified bioluminescent Staphylococcus aureus — a pathogenic bacteria. The bacterial burden at the infection site and the neutrophil recruitment dynamics can be studied using whole animal...
958
Oral Bacterial Infection and Shedding in Drosophila melanogaster09:32

Oral Bacterial Infection and Shedding in Drosophila melanogaster

This protocol describes methods to orally expose and infect the fruit fly Drosophila melanogaster with bacterial pathogens, and to measure the number of infectious bacteria shed following gut infection. We further describe the effect of immune mutants on fly survival following oral bacterial...
12.4K
Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes14:05

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Listeria monocytogenes is a Gram positive bacterial pathogen frequently used as a major model for the study of intracellular parasitism. Imaging late L. monocytogenes infection stages within the context of small-interfering RNA screens allows for the global study of cellular pathways required for bacterial infection of target host...
10.3K
Infecting Flies with Bacterial Suspension Using a Nanoinjector03:37

Infecting Flies with Bacterial Suspension Using a Nanoinjector

This video demonstrates the nano-injection of bacterial suspension into the fly body cavity, resulting in bacterial multiplication and infection. The fly's immune response clears some bacteria, while others escape immune clearance and survive within the host causing an...
459
Using Luciferase to Image Bacterial Infections in Mice10:23

Using Luciferase to Image Bacterial Infections in Mice

Methods for bioluminescence imaging of bacterial infections in living animals are decribed. Pathogens are modified to express luciferase allowing optical whole body imaging of infections in live animals. Animal models can be infected with luciferase expressing pathogens and the resulting course of disease visualized in real-time by bioluminescence...
21.0K