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The T-lymphocyte as a diploid reference standard for flow cytometry.
Cancer Research
|November 1, 1979
Summary
Normal T-lymphocytes provide a stable DNA reference standard for flow cytometry. This method allows for precise detection of abnormal DNA content in cells, aiding in disease diagnosis.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- T-lymphocytes from healthy individuals have consistent DNA content.
- Standardized methods are crucial for accurate cell analysis.
- Flow cytometry is a key technique in cellular research.
Purpose of the Study:
- To establish T-lymphocytes as a reliable external standard for DNA content analysis.
- To assess the stability of T-lymphocytes for use as reference standards.
- To evaluate the sensitivity of flow cytometry in detecting DNA abnormalities.
Main Methods:
- Peripheral blood T-lymphocytes were fixed (ethanol/mithramycin or formalin/acriflavine-Feulgen).
- DNA content was analyzed using flow microfluorometry.
- T-lymphocyte stability was tested during storage.
Main Results:
- T-lymphocytes showed uniform postmitotic DNA content across individuals.
- Fixed T-lymphocytes remained stable for several months.
- Flow cytometry could detect DNA content abnormalities exceeding 5% and less than 5% with multiparameter analysis.
Conclusions:
- T-lymphocytes serve as a stable diploid reference standard for flow cytometry.
- This standardization enhances the accuracy of detecting abnormal DNA content.
- The method is valuable for distinguishing normal from malignant cells based on DNA content.