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Related Concept Videos

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris09:46

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

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The protocol describes protein expression using the methylotrophic yeast Pichia pastoris. The preparation of electrocompetent yeast cells, transformation of the vector with the gene of interest into P. pastoris and yeast DNA purification are also performed. Western blot analysis and protein purification build the last steps in this protein expression protocol.
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Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System08:57

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The baculovirus expression vector system (BEVS) is a robust platform for expression screening and production of protein arginine methyltransferases (PRMTs) to be used for biochemical, biophysical, and structural studies. Milligram quantities of material can be produced for the majority of PRMTs and other proteins of interest requiring a eukaryotic expression platform.
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Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System12:18

Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System

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Here we describe a way to express correctly folded and functional influenza virus surface antigens derived from the novel Chinese H7N9 virus in insect cells. The technique can be adapted to express ectodomains of any viral or cellular surface...
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Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins07:50

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

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Plants offer a novel system for the production of pharmaceutical proteins on a commercial scale that is more scalable, cost-efficient and safe than current expression paradigms. In this study, we report a simple and convenient, yet scalable approach to introduce target-gene containing Agrobacterium tumefaciens into plants for protein transient...
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Production and Optimization of LTE, a Leishmania tarentolae Derived Cell-Free Protein Expression System for Recombinant Protein Production03:59

Production and Optimization of LTE, a Leishmania tarentolae Derived Cell-Free Protein Expression System for Recombinant Protein Production

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Leishmania Translational Extract (LTE) is a eukaryotic cell-free protein expression system derived from the single-celled parasite, Leishmania tarentolae. This optimized protocol makes LTE simple and cost-effective to manufacture. It is suitable for various applications focused on the multiparallel expression and study of complex eukaryotic proteins and their...
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Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

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Generation of recombinant rotaviruses from plasmid DNA provides an essential tool for the study of rotavirus replication and pathogenesis, and the development of rotavirus expression vectors and vaccines. Herein, we describe a simplified reverse genetics approach for generating recombinant rotaviruses, including strains expressing fluorescent reporter...
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Related Experiment Video

Updated: Jan 19, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
09:46

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

Published on: February 25, 2010

47.1K

Special Issue: Recombinant Protein Expression in Microorganisms.

Trygve Brautaset1, Svein Valla2

  • 1Department of Biotechnology and Food Sciences, Norwegian University of Science and Technology (NTNU), Sem Selands vei 6/8, 7491 Trondheim, Norway. trygve.brautaset@ntnu.no.

Microorganisms
|September 19, 2019
PubMed
Summary
This summary is machine-generated.

Microorganisms serve as cell factories in industrial biotechnology for sustainable chemical production. This research explores their potential in creating valuable compounds efficiently.

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Last Updated: Jan 19, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
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Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

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Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
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Area of Science:

  • Industrial biotechnology
  • Microbial cell factories
  • Sustainable chemical synthesis

Background:

  • Microorganisms are pivotal in industrial biotechnology, functioning as 'cell factories' for sustainable compound and chemical production.
  • The inherent metabolic capabilities of microbes enable the biosynthesis of diverse molecules, driving innovation in green chemistry.

Discussion:

  • Exploring the optimization of microbial strains for enhanced production yields.
  • Investigating novel bioprocesses for efficient and cost-effective manufacturing.
  • Addressing challenges in scaling up microbial production for industrial applications.

Key Insights:

  • Microbial cell factories offer a sustainable alternative to traditional chemical synthesis.
  • Metabolic engineering and synthetic biology are key tools for enhancing microbial production.
  • Diverse applications exist, from biofuels to pharmaceuticals and fine chemicals.

Outlook:

  • Future research will focus on expanding the repertoire of microbial products.
  • Advancements in omics technologies and artificial intelligence will accelerate strain development.
  • The role of microorganisms in a circular bioeconomy is expected to grow significantly.