A novel conditional ZsGreen-expressing transgenic reporter rat strain for validating Cre recombinase expression
Elizabeth C Bryda1,2,3,4, Hongsheng Men5,6, Daniel J Davis7
1Rat Resource and Research Center, University of Missouri, Columbia, Missouri, United States of America. brydae@missouri.edu.
Abstract:
The Cre/loxP recombination system has revolutionized the ability to genetically manipulate animal genomes in order to conditionally control gene expression. With recent advances in genome editing, barriers to manipulating the rat genome have been overcome and it is now possible to generate new rat strains (Cre drivers) in which Cre recombinase expression is carefully controlled temporally and/or spatially. However, the ability to evaluate and characterize these Cre driver strains is limited by the availability of reliable reporter rat strains. Here, we describe the generation and characterization of a new transgenic rat strain in which conditional expression of the ZsGreen fluorescent protein gene requires the presence of exogenous Cre recombinase. Breeding Cre-expressing rat strains to this stable ZsGreen reporter strain provides an ideal method for validating new rat Cre driver lines and will greatly accelerate the characterization pipeline.
Related Concept Videos
08:22Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference
10:30Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
12:02Molecular Evolution of the Tre Recombinase
07:06Principles of Site-Specific Recombinase (SSR) Technology
03:12CreER-LoxP System-Based Target Gene Inactivation: A Tamoxifen-Inducible Cre Recombinase System for Target Gene Knockout in a Mouse Model
10:34Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells


