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Related Concept Videos

Feedback Inhibition00:46

Feedback Inhibition

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Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
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Cell Signaling Feedback Loops01:07

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Positive and negative feedback loops are crucial for regulating biological signaling systems. These feedback loops are processes that connect output signals to their inputs.
Negative feedback loops
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Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...
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Here we present a protocol to construct a pressure-controlled syringe pump to be used in microfluidic applications. This syringe pump is made from an additively manufactured body, off-the-shelf hardware, and open-source electronics. The resulting system is low-cost, straightforward to build, and delivers well-regulated fluid flow to enable rapid microfluidic...
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A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments12:21

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We have developed an automated cell culture and interrogation platform for micro-scale cell stimulation experiments. The platform offers simple, versatile, and precise control in cultivating and stimulating small populations of cells, and recovering lysates for molecular analyses. The platform is well suited to studies that use precious cells and/or...
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We describe a nanomoulding technique which allows low-cost nanoscale patterning of functional materials, materials stacks and full devices. Nanomoulding can be performed on any nanoimprinting setup and can be applied to a wide range of materials and deposition...
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Feedback Loops01:01

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In most cases, excessive hormone production is prevented by negative feedback—a loop that starts with a stimulus inducing the release of a particular substance, like a hormone, to maintain a certain level before triggering a signal that results in a decrease in further release of the hormone.
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Related Experiment Video

Updated: Jan 19, 2026

Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications
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Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications

Published on: August 30, 2018

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Versatile biomanufacturing through stimulus-responsive cell-material feedback.

Zhuojun Dai1,2, Anna J Lee1, Stefan Roberts1

  • 1Department of Biomedical Engineering, Duke University, Durham, NC, USA.

Nature Chemical Biology
|September 19, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a microbial swarmbot platform for small-scale biologics production. This cell-material feedback system enables versatile protein production, analysis, and purification, enhancing biomanufacturing accessibility.

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Related Experiment Videos

Last Updated: Jan 19, 2026

Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications
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Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Materials Science

Background:

  • Small-scale biologics production is crucial for improving biomanufacturing accessibility.
  • Current methods may lack versatility and efficiency for diverse protein production.

Purpose of the Study:

  • To design a concise and versatile platform for the production, analysis, and purification of proteins and protein complexes.
  • To leverage cell-material feedback for engineered biomanufacturing.

Main Methods:

  • Development of a microbial swarmbot platform using stimulus-sensitive polymeric microcapsules encapsulating engineered bacteria.
  • Utilizing programmed bacterial lysis and microcapsule shrinkage for protein product release and squeezing.
  • Integration with downstream modules for analysis and purification.

Main Results:

  • Demonstrated versatile production, analysis, and purification of diverse proteins and protein complexes.
  • Enabled quantification of enzymatic kinetics and controlled protein interactions.
  • Facilitated assembly of functional protein complexes and multienzyme metabolic pathways.

Conclusions:

  • The cell-material feedback system engineers a modular and flexible platform for biomanufacturing.
  • This technology offers sophisticated, programmed functions for advanced biological applications.
  • The platform enhances the accessibility and versatility of small-scale biologics production.