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Related Concept Videos

Label-Retention Expansion Microscopy (LR-ExM) Enables Super-Resolution Imaging and High-Efficiency Labeling07:44

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Related Experiment Video

Updated: Jan 19, 2026

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling
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Super-resolution labelling with Action-PAINT.

Ninning Liu1, Mingjie Dai2,3, Sinem K Saka1

  • 1Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.

Nature Chemistry
|September 19, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed Action-PAINT, a super-resolution microscopy technique. This method allows precise, real-time labeling and manipulation of single molecules, advancing nanoscale imaging and molecular studies.

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Last Updated: Jan 19, 2026

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Area of Science:

  • Molecular Biology
  • Microscopy
  • Nanotechnology

Background:

  • Super-resolution microscopy enables visualization of nanoscale molecular features (~5 nm).
  • Current methods lack user-prescribed, context-dependent manipulation of single molecules.
  • Need for precise, real-time molecular labeling and manipulation in biological systems.

Purpose of the Study:

  • To introduce and validate the Action-PAINT strategy for super-resolution labeling.
  • To enable user-prescribed, context-dependent manipulation of single molecules.
  • To achieve real-time, targeted nanoscale labeling and molecular manipulation.

Main Methods:

  • Developed the 'Action-PAINT' (point accumulation for imaging in nanoscale topography) strategy.
  • Utilized DNA-PAINT for real-time monitoring and localization of DNA binding events.
  • Employed 3-cyanovinylcarbazole nucleoside for photo-inducible crosslinking of DNA labels.
  • Created software for real-time super-resolution imaging and crosslinking control.

Main Results:

  • Demonstrated efficient photo-crosslinking of single molecular targets.
  • Achieved targeted multipoint labeling on DNA nanostructures with 30 nm selectivity.
  • Successfully performed in situ labeling on fixed microtubule samples with 40 nm target size and controlled subdiffraction spacing.

Conclusions:

  • Action-PAINT provides a novel method for super-resolution labeling and manipulation of single molecules.
  • The technique allows for precise, real-time control over molecular labeling in complex biological samples.
  • This approach significantly advances nanoscale imaging and molecular manipulation capabilities.