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Related Concept Videos

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma07:55

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma

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The following protocol focuses on the establishment of a primary culture of patient-derived soft tissue sarcoma (STS). This model could help us to better understand the molecular background and pharmacological profile of these rare malignancies and could represent a starting point for further research aimed at improving STS...
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Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus (KSHV)07:02

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Host cell factors play a critical role in the establishment and maintenance of Kaposi's sarcoma (KS). We outline methods to identify host cell factors altered in KSHV-infected DMVEC cells, and in KS tumor tissue. Cellular genes altered by virus will serve as potential target(s) for novel...
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The In ovo CAM-assay as a Xenograft Model for Sarcoma12:44

The In ovo CAM-assay as a Xenograft Model for Sarcoma

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The in ovo chorioallantoic membrane (CAM) is grafted with fresh sarcoma-derived tumor tissues, their single cell suspensions, and permanent and transient fluorescently labeled established sarcoma cell lines. The model is used to study graft- (viability, Ki67 proliferation index, necrosis, infiltration) and host (fibroblast infiltration, vascular ingrowth)...
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Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

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We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and...
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An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus09:53

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Kaposi sarcoma (KS) is a tumor induced by infection with the oncogenic virus human herpesvirus-8/KS herpesvirus (HHV-8/KSHV). The endothelial cell culture model described here is uniquely suited for studying the mechanisms by which KSHV transforms host cells.
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Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

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We describe a detailed protocol for the isolation of tumor-initiating cells from human sarcoma patient-derived xenografts by fluorescence-activated cell sorting, using human leukocyte antigen-1 (HLA-1) as a negative marker, and for the further validation and characterization of these HLA-1-negative tumor-initiating...
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Related Experiment Video

Updated: Jan 19, 2026

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
07:55

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma

Published on: April 11, 2018

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Primary pulmonary undifferentiated pleomorphic sarcoma (PPUPS).

Amir Qorbani1, Scott D Nelson2

  • 1University of California, San Francisco (UCSF), UCSF Medical Center at Mission Bay. San Francisco, CA, United States of America.

Autopsy & Case Reports
|September 19, 2019
PubMed
Summary
This summary is machine-generated.

Primary Pulmonary Undifferentiated Pleomorphic Sarcoma (PPUPS) is a rare lung cancer. This study presents a case and reviews 84 prior cases, offering insights into this challenging diagnosis.

Keywords:
LungLung NeoplasmsSarcomaSoft Tissue Neoplasms

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The In ovo CAM-assay as a Xenograft Model for Sarcoma
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Related Experiment Videos

Last Updated: Jan 19, 2026

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
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Published on: April 11, 2018

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Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus KSHV
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Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus KSHV

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The In ovo CAM-assay as a Xenograft Model for Sarcoma
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Area of Science:

  • Oncology
  • Pathology

Background:

  • Undifferentiated pleomorphic sarcoma (UPS) is a high-grade neoplasm lacking identifiable differentiation markers.
  • It is a diagnosis of exclusion, necessitating comprehensive diagnostic evaluation.
  • Pulmonary involvement is typically metastatic, making primary lung UPS exceptionally rare.

Observation:

  • A 66-year-old male presented with anemia and weight loss.
  • A large (17 cm) right lung mass invading the chest wall and diaphragm was identified.
  • Extensive sampling and immunohistochemistry failed to reveal a specific line of differentiation.

Findings:

  • A diagnosis of Primary Pulmonary Undifferentiated Pleomorphic Sarcoma (PPUPS) was established after excluding extrapulmonary origins.
  • A review of 84 previously reported PPUPS/PPMFH cases since 1979 was conducted.
  • Clinical information from these cases was summarized.

Implications:

  • This case highlights the rarity and diagnostic challenges of PPUPS.
  • Understanding the clinical characteristics of PPUPS is crucial for early detection and management.
  • Further research may elucidate specific diagnostic markers or therapeutic strategies for PPUPS.