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Updated: Jan 19, 2026

Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System
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Generation and Validation of Tissue-Specific Knockout Strains for Toxicology Research

Cherish A Taylor1, William Shawlot2, Jin Xiang Ren2

  • 1Division of Pharmacology & Toxicology, College of Pharmacy, Institute for Cellular & Molecular Biology and Institute for Neuroscience, University of Texas at Austin, Austin, Texas.

Current Protocols in Toxicology
|September 19, 2019
PubMed
Summary

This study details the Cre-loxP system for generating tissue-specific knockout mice. It covers creating conditional alleles, breeding strategies, and validation methods for reliable genetic research models.

Keywords:
Cre-loxPknockout micetissue-specific

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