Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dopamine receptors in the developing sheep kidney.

R A Felder1, K T Nakamura, J E Robillard

  • 1Department of Pathology, University of Virginia Medical Center, Charlottesville.

Pediatric Nephrology (Berlin, Germany)
|January 1, 1988
PubMed
Summary

Kidney dopamine-1 receptor density remains stable across sheep ages. However, dopamine-2 receptor density in sheep kidneys significantly decreases with age, suggesting developmental changes in renal dopaminergic systems.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Drug Repurposing: An Effective Tool in Modern Drug Discovery.

Russian journal of bioorganic chemistry·2023
Same author

Transient depletion of macrophages alters local inflammatory response at the site of disc herniation in a transgenic mouse model.

Osteoarthritis and cartilage·2023
Same author

In vivo predation and modification of the Mediterranean fruit fly Ceratitis capitata (Wiedemann) gut microbiome by the bacterial predator Bdellovibrio bacteriovorus.

Journal of applied microbiology·2021
Same author

Dopaminergic Immunofluorescence Studies in Kidney Tissue.

Methods in molecular biology (Clifton, N.J.)·2017
Same author

Secreted Monocyte miR-27a Causes Hypertension by Reducing Mas Receptor Expression and Function in the Artery.

Journal of the American Society of Hypertension : JASH·2016
Same author

Role of GRK4 in the Regulation of Arterial AT1 Receptor in Hypertension.

Journal of the American Society of Hypertension : JASH·2016

Area of Science:

  • Nephrology
  • Neuropharmacology
  • Physiology

Background:

  • Dopamine receptors play a role in regulating renal function.
  • Understanding age-related changes in renal dopamine receptors is crucial for comprehending kidney physiology.

Purpose of the Study:

  • To investigate age-dependent alterations in dopamine receptor density and affinity within sheep kidneys.
  • To characterize specific dopamine-1 and dopamine-2 receptor binding across different developmental stages.

Main Methods:

  • Radioligand binding assays were employed using three specific radioligands: 3H-SCH 23390 (dopamine-1 antagonist), 3H-haloperidol (dopamine-1/dopamine-2 antagonist), and 3H-spiroperidol (dopamine-2 antagonist).
  • Kidney tissues from fetal, newborn, and adult sheep were analyzed.
  • Saturation binding kinetics were determined to assess receptor density and affinity.

Related Experiment Videos

Main Results:

  • Specific binding of 3H-haloperidol and 3H-spiroperidol indicated the presence of saturable dopamine receptors.
  • Dopamine-1 receptor density and affinity, measured by 3H-haloperidol, showed no significant age-related changes.
  • Maximum receptor density for dopamine-2 receptors, assessed using 3H-spiroperidol, demonstrated a decrease with advancing age.

Conclusions:

  • Renal dopamine-1 receptor characteristics remain consistent throughout sheep maturation.
  • A significant decline in renal dopamine-2 receptor density occurs with age in sheep.
  • The functional implications of decreased dopamine-2 receptor density in aging sheep kidneys warrant further investigation.