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Updated: Jan 19, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
[The immune-enzyme techniques of analysis in diagnostic of cholera.]
V V Evdokimova1, L P Alekseeva1, O F Kretentchuk1
1The Rostov-on-Don research anti-plague institute of Rospotrebnadzor, 344002, Rostov-on-Don, Russia.
Abstract:
The optimal conditions of arrangement of direct alternative of flatbed enzyme-linked immunosorbent assay and dot-immunoanalysis with application of monoclonal peroxidase conjugates for express identification of comma bacillus of serogroups O1 and O139 both in hospital and field conditions without device support. The direct technique enzyme-linked immunosorbent assay in flatbed alternative shortens time of analysis up to 70-80 minutes and in case of dot enzyme-linked immunosorbent assay on membrane - up to 70-90 minutes. It is established that in case of analysis in conditions of room temperature (20-25 oC) sensitivity of techniques remains at initial level.
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