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Overexpressed Tomosyn Binds Syntaxins and Blocks Secretion during Pollen Development.

Bingxuan Li1, Yanbin Li1, Feng Liu1

  • 1State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.

Plant Physiology
|September 19, 2019
PubMed
Summary
This summary is machine-generated.

Arabidopsis Tomosyn protein (AtTMS) regulates syntaxins (SYPs) in plants, inhibiting secretion and causing pollen defects when overexpressed. This protein acts as a negative regulator, fine-tuning secretion during pollen development.

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Area of Science:

  • Plant molecular biology
  • Cellular secretion mechanisms
  • Membrane fusion regulation

Background:

  • SNARE complex formation is crucial for intracellular membrane fusion and secretion.
  • Regulatory factors binding to plant Qa-SNAREs (syntaxins/SYPs) are not well understood.

Purpose of the Study:

  • To characterize Arabidopsis Tomosyn protein (AtTMS) as a conserved regulator of plant SYPs.
  • To investigate the role of AtTMS in pollen development and secretion.

Main Methods:

  • Yeast two-hybrid screening to identify SYP1s interacting with AtTMS.
  • Analysis of AtTMS expression patterns in Arabidopsis pollen.
  • Overexpression studies of AtTMS in Arabidopsis and tobacco plants.
  • Rescue experiments using SYP variants.

Main Results:

  • AtTMS binds to the Qa domain of pollen-expressed SYP1s (SYP111, SYP124, SYP125, SYP131, SYP132).
  • Overexpression of AtTMS in Arabidopsis leads to defective pollen with inhibited secretion, affecting intine deposition and cell plate formation.
  • Overexpression of AtTMS in tobacco inhibits secretion, which can be rescued by specific SYP1s.
  • AtTMS competes with VAMP721/722 for SYP binding and is recruited to the plasma membrane by SYPs.

Conclusions:

  • AtTMS acts as a conserved negative regulator of secretion in plants.
  • AtTMS plays a critical role in fine-tuning secretion during pollen development.
  • AtTMS interacts with SYPs to control membrane fusion and secretion processes.