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Fabrication of the Thermoplastic Microfluidic Channels16:00

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Here we demonstrate how to fabricate thermoplastic microfluidic chips using hot embossing and heat sealing. Then we demonstrate how to use in situ light directed surface grafting and polymerization through the sealed chip to form the composite solid phase...
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Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos08:50

Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos

Here, we describe a method for warming vitrified human blastocysts, culturing them through the implantation period in vitro, digesting them into single cells and collecting early trophoblast cells for further...
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A protocol outlining the formation of human blastoids that efficiently, timely, and sequentially generate blastocyst-like...
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Microbead Implantation in the Zebrafish Embryo

The zebrafish is an excellent model system for genetic and developmental studies. Bead implantation is a valuable tissue manipulation technique that can be used to interrogate developmental mechanisms by introducing alterations in local cellular environments. This protocol describes how to perform microbead implantation in the zebrafish...
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Reconfigurable Microfluidic Channel with Pin-discretized Sidewalls10:39

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A microfluidic channel with deformable sidewalls offers flow control, particle handling, channel dimension customization and other reconfigurations while in use. We describe a method for fabricating a microfluidic channel with sidewalls made of an array of pins that allows their shape to...
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