Related Experiment Video
Updated: Jan 19, 2026

Following Cell-fate in E. coli After Infection by Phage Lambda
Published on: October 14, 2011
Complete genome sequence of the novel phage vB_EcoS_PHB17, which infects Shiga-toxin-producing Escherichia coli
Yibao Chen1,2, Shuang Wang1,2, Yunqing Wang1,2
1State Key laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Abstract:
The complete genome of the novel phage vB_EcoS_PHB17, which infects Shiga-toxin-producing Escherichia coli, was sequenced, revealing a linear double-stranded DNA genome of 48,939 bp with 46% GC content and protruding 150-bp 5' cohesive termini. The genome contained 85 open reading frames, 28 of which were annotated with known functions. No tRNA-encoding genes were detected. Phylogenetic analysis suggested that phage PHB17 is a novel phage of family Siphoviridae.
More Related Videos
Related Concept Videos
06:10Following Cell-fate in E. coli After Infection by Phage Lambda
09:44Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
08:27Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat
Phage Transduction: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli
1 Department of Microbiology, Boston University School of Medicine, National Emerging Infections Diseases Laboratories, Boston, MA
Transduction is a form of genetic exchange between bacteria that utilizes bacteriophages, or phages, a class of virus that infects exclusively prokaryotic organisms. This form of DNA transfer, from one bacterium to another by way of a phage, was discovered in 1951 by Norton Zinder and Joshua Ledererg, who termed the...
08:13Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes
10:52Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

