Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens04:54

Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

41.5K
A simple and efficient method to transform Physcomitrella pantens protoplasts is described. This method is adapted from protocols for Physocmitrella protonemal protoplast and Arabidopsis mesophyll protoplast...
41.5K
Bacterial Transformation: Electroporation12:19

Bacterial Transformation: Electroporation

119.2K
The term “transformation” refers cellular ingestion of foreign DNA. In nature, transformation can occur in certain types of bacteria. In molecular biology, however, transformation is artificially induced through the creation of pores in the bacterial cell walls. Bacterial cells that are able to take up DNA from the environment are called competent cells. Electrocompetent cells can be produced in the laboratory and transformation of these cells can be achieve via the...
119.2K
Bacterial Transformation05:21

Bacterial Transformation

26.6K
Bacterial Transformation
ExpandIMPORTANT: In addition to wearing the appropriate personal protective equipment, be sure to take care to keep your face away from suspension cultures, and to avoid inhaling reagents. Do not touch your face while performing the experiment. Always wash your hands before and after every experiment.
When you are ready to safely begin, check to make sure you have a tube or tubes of competent E. coli cells in your ice bucket.
Then, transfer 50 µL from one of these...
26.6K
The Resting Membrane Potential01:21

The Resting Membrane Potential

141.9K
Overview
141.9K
Bacterial Transformation01:33

Bacterial Transformation

59.5K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
59.5K
Resting Membrane Potential01:24

Resting Membrane Potential

21.4K
The relative difference in electrical charge, or voltage, between the inside and the outside of a cell membrane, is called the membrane potential. It is generated by differences in permeability of the membrane to various ions and the concentrations of these ions across the membrane.
The Inside of a Neuron is More Negative
The membrane potential of a cell can be measured by inserting a microelectrode into a cell and comparing the charge to a reference electrode in the extracellular fluid. The...
21.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Increased paclitaxel recovery from Taxus baccata vascular stem cells using novel in situ product recovery approaches.

Bioresources and bioprocessing·2024
Same author

Remote Activation of Enzyme Nanohybrids for Cancer Prodrug Therapy Controlled by Magnetic Heating.

ACS nano·2023
Same author

Bacteria-Polymer Composite Material for Glycerol Valorization.

Polymers·2023
Same author

New perspectives into Gluconobacter-catalysed biotransformations.

Biotechnology advances·2023
Same author

Dihydroxyacetone production via heterogeneous biotransformations of crude glycerol.

Journal of biotechnology·2021
Same author

Stabilization of ω-transaminase from Pseudomonas fluorescens by immobilization techniques.

International journal of biological macromolecules·2020

Related Experiment Video

Updated: Jul 6, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

Efficient glycerol transformation by resting Gluconobacter cells.

Erienne Jackson1, Magdalena Ripoll1, Lorena Betancor1

  • 1Department of Biotechnology, Universidad ORT Uruguay, Montevideo, Uruguay.

Microbiologyopen
|September 19, 2019
PubMed
Summary

This study optimized glycerol biotransformation using Gluconobacter strains for cleaner production. Resting cells efficiently converted crude glycerol into dihydroxyacetone (DHA) with unprecedented productivity in water alone.

Keywords:
Gluconobacterbiotransformationdihydroxyacetoneglyceric acidglycerol

More Related Videos

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
05:30

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Published on: January 23, 2019

Related Experiment Videos

Last Updated: Jul 6, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
05:30

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Published on: January 23, 2019

Area of Science:

  • Biotechnology
  • Industrial Microbiology
  • Biochemical Engineering

Background:

  • Glycerol biotransformation is a key process for valorizing industrial by-products.
  • Existing methods often involve complex media and lower efficiencies.
  • Process intensification is needed for cleaner and more sustainable glycerol conversion.

Purpose of the Study:

  • To develop a cleaner and more efficient technology for glycerol biotransformation.
  • To investigate the bioconversion of crude glycerol using Gluconobacter strains.
  • To achieve high productivities of valuable compounds from glycerol.

Main Methods:

  • Utilized resting cells of Gluconobacter frateurii and Gluconobacter oxydans.
  • Employed batch reactor conditions with crude glycerol in water.
  • Optimized parameters including strain, biomass ratio, pH, growth stage, and media composition.

Main Results:

  • Achieved high productivities for glyceric acid (0.03 g/L.h) and dihydroxyacetone (DHA) (2.07 g/L.h).
  • DHA purity reached 71.5 g/g % as determined by NMR.
  • Productivities surpassed previously reported fermentative bioconversions and were unprecedented in water alone.

Conclusions:

  • Developed a novel, intensified approach for glycerol biotransformation.
  • Demonstrated significantly higher productivities and cleaner production.
  • Reduced water and energy consumption, showcasing practical applicability.