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Updated: Jan 19, 2026

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Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
Published on: February 26, 2015
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[Three-step sequential method for limb skin and soft tissue defect combined with bone or tendon exposed wound]
Wei-Bin Du1, Li-Xiang Wang2, Guo-Ming Wu1
1Department of Orthopaedics, Jiangnan Hospital Affiliated to Zhejiang University of Chinese Medicine, Hangzhou 311201, Zhejiang, China.
Zhongguo Gu Shang = China Journal of Orthopaedics and Traumatology
|September 20, 2019
Summary
The three-step sequential method effectively treats limb soft tissue defects with exposed bone or tendon. This approach utilizes VSD technology, artificial dermis, and autografting for successful wound healing and good cosmetic outcomes.
Area of Science:
- Reconstructive surgery
- Tissue engineering
- Wound healing
Background:
- Limb skin soft tissue defects with exposed bone or tendon pose significant reconstructive challenges.
- Traditional flap repair may not always be suitable or desired by patients.
Purpose of the Study:
- To evaluate the clinical efficacy of a three-step sequential method using VSD technology, artificial dermis, and autograft for treating limb soft tissue defects with exposed bone or tendon.
- To assess wound recovery, graft survival, and aesthetic outcomes.
Main Methods:
- A cohort of 18 patients with limb skin soft tissue defects and exposed bone or tendon underwent a three-step sequential treatment.
- The method involved VSD technology, artificial dermis coverage, and subsequent autografting.
- Patients were followed up for wound healing and graft take.
Main Results:
- All treated wounds showed effective recovery of deep bone and tendon tissues.
- The artificial dermis demonstrated successful survival, leading to tough, well-shaped healed wounds.
- Autograft survival was good, with satisfactory cosmetic results including flat appearance and good skin color match.
Conclusions:
- The three-step sequential method is a viable and effective treatment for limb soft tissue defects with exposed bone or tendon, especially for patients who decline flap repair.
- This technique offers advantages of simple operation, low surgical risk, and minimally invasive characteristics.

