Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dependence of lymphocyte surface Ig on continuous polyclonal activation.

D M Bucholz, S Dray, M Teodorescu

    Immunology
    |August 1, 1979
    PubMed
    Summary

    Rabbit B lymphocytes shed and replace surface immunoglobulin (Ig) with a half-life of approximately 2 hours. This replacement, crucial for maintaining Ig levels, depends on continuous polyclonal activation, either external or internal.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    New organophilic kaolin clays based on single-point grafted 3-aminopropyl dimethylethoxysilane.

    Physical chemistry chemical physics : PCCP·2015
    Same author

    Intrapericardial paraganglioma.

    JBR-BTR : organe de la Societe royale belge de radiologie (SRBR) = orgaan van de Koninklijke Belgische Vereniging voor Radiologie (KBVR)·2014
    Same author

    Considering external information to improve the phylogenetic comparison of microbial communities: a new approach based on constrained Double Principal Coordinates Analysis (cDPCoA).

    Molecular ecology resources·2014
    Same author

    Liposome-Mediated transfer of bacterial RNA into carrot protoplasts.

    Planta·2013
    Same author

    Evaluation of different immunoassays for the detection of antiphospholipid antibodies: report of a wet workshop during the 13th International Congress on Antiphospholipid Antibodies.

    Clinica chimica acta; international journal of clinical chemistry·2013
    Same author

    Physio-chemical hydrodynamic mechanism underlying the formation of thin adsorbed boundary films.

    Faraday discussions·2013

    Area of Science:

    • Immunology
    • Cell Biology
    • Molecular Biology

    Background:

    • Rabbit splenic B cells require continuous polyclonal stimulation to maintain surface immunoglobulin (Ig).
    • Without stimulation, B cells shed surface Ig but do not replace it, leading to loss.

    Purpose of the Study:

    • To investigate the mechanism behind the loss or maintenance of surface Ig on rabbit B cells.
    • To determine if mitogen stimulation affects surface Ig by preventing shedding or promoting resynthesis.

    Main Methods:

    • Cultured rabbit splenic B cells with and without mitogens (B-cell specific: SM, LPS; T-cell specific: Con A, PHA).
    • Used inhibitors of mRNA and protein synthesis to assess the role of resynthesis.
    • Labeled surface Ig with 125I-labelled Fab anti-allotype antibody to track turnover.

    Related Experiment Videos

  • Cultured cells under crowded conditions to investigate endogenous factors.

    • Main Results:

    • Inhibitors of mRNA and protein synthesis abolished mitogen-induced Ig maintenance, indicating resynthesis is required.
    • Mitogen stimulation led to surface Ig turnover (shedding and replacement) with a half-life of ~2 hours.
    • B-cell mitogens maintained surface Ig, while T-cell mitogens did not, suggesting direct B-cell activation is necessary.
    • Crowded conditions induced a macroglobulin-associated factor that maintained surface Ig turnover, mimicking polyclonal activation.

    Conclusions:

    • Rabbit B lymphocytes shed and replace surface Ig with a half-life of approximately 2 hours.
    • Surface Ig replacement, not shedding, is dependent on continuous exogenous or endogenous polyclonal activation.
    • Direct B-cell stimulation is essential for maintaining surface Ig levels.