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Draft Genome Sequence of Multidrug-Resistant Proteus mirabilis CKTH01, Isolated from Raw Chicken Meat
Supatra Areekit1,2, Nuttida Thongpramul3, Wariya Yamprayoonswat3
1Innovative Learning Center, Srinakharinwirot University, Bangkok, Thailand.
Abstract:
Proteus mirabilis CKTH01 is a pathogenic bacterium isolated from raw chicken meat. The genome sequence of P. mirabilis CKTH01 contains genes encoding multidrug efflux pumps, which are the virulence factors of the antibiotic-resistant bacterium. This 3.98-Mb draft genome sequence of P. mirabilis CKTH01 will contribute to the understanding of the distribution of multidrug-resistant P. mirabilis in raw chicken meat at the open markets.
Insights
The genome of the pathogenic bacterium Proteus mirabilis CKTH01, found in raw chicken, reveals multidrug efflux pump genes. This finding aids in understanding antibiotic-resistant bacteria distribution in markets.
Area of Science:
- Microbiology
- Genomics
- Food Safety
Background:
- Proteus mirabilis is a pathogenic bacterium.
- Multidrug efflux pumps are key virulence factors in antibiotic-resistant bacteria.
- Raw chicken meat can be a source of foodborne pathogens.
Purpose of the Study:
- To sequence the draft genome of Proteus mirabilis CKTH01.
- To identify genes encoding multidrug efflux pumps.
- To understand the prevalence of antibiotic-resistant P. mirabilis in raw chicken.
Main Methods:
- Isolation of Proteus mirabilis CKTH01 from raw chicken meat.
- Whole-genome sequencing of the isolate.
- Bioinformatic analysis to identify virulence genes, specifically multidrug efflux pumps.
Main Results:
- A 3.98-Mb draft genome sequence for P. mirabilis CKTH01 was obtained.
- Genes encoding multidrug efflux pumps were identified within the genome.
- These pumps are associated with the bacterium's antibiotic resistance and virulence.
Conclusions:
- The genome sequence provides insights into the genetic basis of antibiotic resistance in P. mirabilis CKTH01.
- Understanding these genetic factors is crucial for tracking and controlling the spread of resistant strains.
- This study contributes to food safety by elucidating the potential for multidrug-resistant P. mirabilis in raw chicken products available in markets.
