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Updated: Jul 31, 2026
Protocol for Dengue Infections in Mosquitoes A. aegypti and Infection Phenotype Determination
Published on: July 4, 2007
Inhibitory Effect of Alpha-Mangostin to Dengue Virus Replication and Cytokines Expression in Human Peripheral Blood
Zaenal Sugiyanto1,2, Benediktus Yohan3, Soeharyo Hadisaputro2,4
1Faculty of Health Science, Dian Nuswantoro University, Jl. Imam Bonjol 270, Semarang, 50131, Indonesia.
Abstract:
Massive pro-inflammatory cytokines production has been correlated with the pathogenesis of severe dengue disease. The active compound of mangosteen fruit pericarps, α-mangostin, has been commonly used as traditional medicine and dietary supplement. We examined the effect of α-mangostin against dengue virus (DENV) infection in human peripheral blood mononuclear cells (PBMC) by the measurement of virus titer and TNF-α and IFN-γ cytokines concentration post infection. Increasing concentration of α-mangostin inhibited virus replication and reduced inflammatory cytokines expression at 24- and 48-h post infection. Our results support the potential use of α-mangostin as anti-antiviral and anti-inflammatory therapies in the treatment of dengue.
Insights
Alpha-mangostin, derived from mangosteen, shows potential as an antiviral and anti-inflammatory treatment for dengue. This compound effectively inhibited dengue virus replication and reduced inflammatory cytokine production in human cells.
Area of Science:
- Virology
- Immunology
- Pharmacology
Background:
- Severe dengue is linked to excessive pro-inflammatory cytokine production.
- Alpha-mangostin, a compound from mangosteen, is a traditional medicine with potential health benefits.
Purpose of the Study:
- To investigate the antiviral and anti-inflammatory effects of alpha-mangostin against dengue virus (DENV) infection.
- To evaluate alpha-mangostin's impact on DENV replication and cytokine production in human cells.
Main Methods:
- Human peripheral blood mononuclear cells (PBMCs) were infected with DENV.
- The effect of varying concentrations of alpha-mangostin on virus titer and cytokine levels (TNF-α, IFN-γ) was measured at 24 and 48 hours post-infection.
Main Results:
- Alpha-mangostin demonstrated dose-dependent inhibition of DENV replication.
- Reduced expression of pro-inflammatory cytokines, TNF-α and IFN-γ, was observed with alpha-mangostin treatment.
- These effects were noted at both 24 and 48 hours post-infection.
Conclusions:
- Alpha-mangostin exhibits significant antiviral activity against DENV in human PBMCs.
- The compound possesses anti-inflammatory properties by reducing key cytokine production.
- Alpha-mangostin shows promise as a therapeutic agent for dengue treatment, potentially mitigating severe disease pathogenesis.
