Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In Vitro Drug Dissolution: Compendial Testing Models II01:09

In Vitro Drug Dissolution: Compendial Testing Models II

266
Various dissolution methods are utilized to assess a drug’s dissolution rate, including the flow-through cell, paddle-over-disk, cylinder, and reciprocating disk methods.The flow-through cell apparatus (USP (United States Pharmacopeia) method 4) comprises a reservoir for the dissolution medium and a pump that propels the medium through the cell containing the test sample. This method is crucial for assessing modified-release dosage forms with minimally soluble active ingredients,...
266
In Vitro Drug Dissolution: Compendial Testing Models I01:13

In Vitro Drug Dissolution: Compendial Testing Models I

239
Compendial dissolution methods are standardized procedures defined by pharmacopeias to evaluate the rate at which a drug dissolves in a specific medium. These methods ensure batch-to-batch consistency, enable quality control, and support the prediction of drug bioavailability. They are critical for both immediate and modified-release drug products.The apparatuses used for dissolution testing differ in their design and mechanical function, but all aim to simulate the physiological environment of...
239
Clinically Relevant Drug Product Specifications: Methods of Establishment01:29

Clinically Relevant Drug Product Specifications: Methods of Establishment

207
Product specifications define the acceptable quality of a pharmaceutical product by ensuring identity, purity, potency, and strength. These specifications serve as benchmarks during development, manufacturing, and post-approval quality control. Clinically relevant specifications are particularly important because they directly relate to a drug's safety and efficacy in clinical use.Dissolution studies are critical biopharmaceutic tools that link in vitro behavior to in vivo performance. They...
207
Drug Product Performance: In Vitro–In Vivo Correlation01:20

Drug Product Performance: In Vitro–In Vivo Correlation

239
In pharmaceutical development, it's crucial to establish a predictive in vitro–in vivo correlation (IVIVC) for two or more formulations to gain a comprehensive understanding of release properties. IVIVC reduces the need for costly in vivo studies and facilitates the establishment of meaningful dissolution specifications with significant cost savings and decreased regulatory burden. Furthermore, a meaningful IVIVC should predict Cmax and AUC within 20%, aligning with FDA guidance while...
239
Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

578
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
578

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring chemical space, scaffold diversity, and activity landscape of spleen tyrosine kinase active inhibitors.

SAR and QSAR in environmental research·2024
Same author

Correction to: Large‑scale pathway specific polygenic risk and transcriptomic community network analysis identifies novel functional pathways in Parkinson disease.

Acta neuropathologica·2021
Same author

Scaling Up Maternal Mental healthcare by Increasing access to Treatment (SUMMIT) through non-specialist providers and telemedicine: a study protocol for a non-inferiority randomized controlled trial.

Trials·2021
Same author

Large-scale pathway specific polygenic risk and transcriptomic community network analysis identifies novel functional pathways in Parkinson disease.

Acta neuropathologica·2020
Same author

Therapist Reports of EBP Client Engagement Challenges in Sessions with Diverse Youth and Families in Community Mental Health Settings.

Child & youth care forum·2020
Same author

Development and validation of a clinical prediction-score model for distant metastases in major salivary gland carcinoma.

Annals of oncology : official journal of the European Society for Medical Oncology·2020
Same journal

Institutional Patriarchy, Gender Barriers, and Nigeria's Oral Health Workforce.

Advances in dental research·2025
Same journal

Sex, Gender and Sexuality in Oral Health Research: Setting the Scene.

Advances in dental research·2025
Same journal

Beyond Demographics: Sex, Gender, and Sexuality in Oral Health Research.

Advances in dental research·2025
Same journal

Conceptualisations of Sex and Gender in Oral Health Research.

Advances in dental research·2025
Same journal

Analysing the Interaction of Sex and Laboratory Conditions in Animal Research.

Advances in dental research·2025
Same journal

Advancing Oral Health Workforce Equity in Gender and Sexual Orientation.

Advances in dental research·2025
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
09:25

Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations

Published on: January 10, 2015

11.8K

In Vitro Models, Standards, and Experimental Methods for Tobacco Products.

T Aghaloo1, J J Kim2, T Gordon3

  • 1Section of Oral and Maxillofacial Surgery, UCLA School of Dentistry, Los Angeles, CA, USA.

Advances in Dental Research
|September 21, 2019
PubMed
Summary
This summary is machine-generated.

New tobacco products like e-cigarettes pose unknown oral health risks. Research methods are needed to study these risks, as current approaches primarily focus on respiratory effects.

Keywords:
inflammationnicotineoral carcinogenesissystemic health/diseasetobaccowound healing

More Related Videos

In vitro Cannabis Exposures of Lung Epithelial Cells at the Air-Liquid Interface
07:05

In vitro Cannabis Exposures of Lung Epithelial Cells at the Air-Liquid Interface

Published on: June 20, 2025

882
A Microcontroller Operated Device for the Generation of Liquid Extracts from Conventional Cigarette Smoke and Electronic Cigarette Aerosol
09:30

A Microcontroller Operated Device for the Generation of Liquid Extracts from Conventional Cigarette Smoke and Electronic Cigarette Aerosol

Published on: January 18, 2018

8.8K

Related Experiment Videos

Last Updated: Jan 19, 2026

Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
09:25

Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations

Published on: January 10, 2015

11.8K
In vitro Cannabis Exposures of Lung Epithelial Cells at the Air-Liquid Interface
07:05

In vitro Cannabis Exposures of Lung Epithelial Cells at the Air-Liquid Interface

Published on: June 20, 2025

882
A Microcontroller Operated Device for the Generation of Liquid Extracts from Conventional Cigarette Smoke and Electronic Cigarette Aerosol
09:30

A Microcontroller Operated Device for the Generation of Liquid Extracts from Conventional Cigarette Smoke and Electronic Cigarette Aerosol

Published on: January 18, 2018

8.8K

Area of Science:

  • Oral health research
  • Tobacco product toxicology

Background:

  • Traditional tobacco use causes significant oral and systemic health issues, including cancer and periodontal disease.
  • Cigarette smoking has declined, but novel tobacco product use, such as electronic cigarettes, is increasing.
  • The oral health consequences of electronic cigarettes are not well understood.

Purpose of the Study:

  • To evaluate current in vitro and in vivo research methods for studying the oral effects of new tobacco products.
  • To highlight the need for developing new tools to assess the oral sequelae of electronic cigarettes.
  • To address the challenges in researching rapidly evolving tobacco product technologies.

Main Methods:

  • Review of existing in vitro toxicologic models and assays for electronic cigarettes, noting their primary focus on respiratory effects.
  • Examination of recent in vitro studies exposing 3-dimensional tissue constructs to electronic cigarette aerosol.
  • Discussion of the need for standardized in vivo testing, including universal e-cigarette testing machines and standard vaping regimes.

Main Results:

  • In vitro studies show electronic cigarette aerosol can impact cell viability and inflammatory cytokine expression in oral tissue constructs.
  • Development of standardized research electronic cigarettes and devices with varying nicotine concentrations is emerging.
  • Keeping pace with new product development and technological advancements in the industry presents a significant research challenge.

Conclusions:

  • There is a critical need to develop and refine research methodologies for evaluating the oral health impacts of electronic cigarettes and other novel tobacco products.
  • Standardized testing protocols and devices are essential for consistent and reliable in vivo research.
  • Continued research is necessary to understand and mitigate the potential oral health risks associated with emerging tobacco product use.