Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

Restarting Stalled Replication Forks

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Tunable Patterning of DNA Origami on Surfaces Using Steric Brushes.

Angewandte Chemie (International ed. in English)·2026

Same author

A synthetic cell microreactor with two types of interacting dynamic DNA-based pores.

Nature chemistry·2026

Same author

Hierarchical Logic Control via DNA Polymerase-Driven Molecular Circuits.

ACS applied bio materials·2026

Same author

Conformation-programmed DNA computing.

Science advances·2026

Same author

Expanding DNA alphabet adds a previously unknown dimension to nanostructures.

Science advances·2026

Same author

Designer RNA nanostructures co-transcribed and self-assembled inside human cell nuclei.

Nature communications·2025

Same journal

Gas-Responsive Metal-Organic Frameworks for Adaptive Thermal Energy Storage with Tunable Charge-Discharge Temperatures.

Journal of the American Chemical Society·2026

Same journal

Engineering a Thiamine-Dependent Benzoylformate Decarboxylase for Stereodivergent Radical C(sp<sup>3</sup>)-C(sp<sup>3</sup>) Bond Formation.

Journal of the American Chemical Society·2026

Same journal

Accelerated Directional Proton-Coupled Electron Transfer Enabled by Intrinsic Dipole Field in Biomimetic α-Helical Structure.

Journal of the American Chemical Society·2026

Same journal

Alternating Current-Driven Hydrogen Isotope Labeling of Aliphatic Amines Using 1,3-Propanedithiol as an Efficient Hydrogen Atom Transfer Reagent.

Journal of the American Chemical Society·2026

Same journal

Two-Dimensional van der Waals Polar Metal MoOBr<sub>2</sub>.

Journal of the American Chemical Society·2026

Same journal

Negatively Curved Chiral Bilayer Nanographene.

Journal of the American Chemical Society·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 19, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Nicking-Assisted Reactant Recycle To Implement Entropy-Driven DNA Circuit.

Cheng Zhang^1,2, Zhiyu Wang³, Yan Liu

¹School of Electronics Engineering and Computer Science , Peking University , Beijing 100871 , China.

Journal of the American Chemical Society

|September 21, 2019

Summary

This summary is machine-generated.

Synthetic DNA circuits enable signal amplification in molecular programming. A new nicking-assisted recycling strategy improves reactant recyclability in entropy-driven DNA circuits, enhancing efficiency for synthetic biology applications.

More Related Videos

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Related Experiment Videos

Last Updated: Jan 19, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Area of Science:

Synthetic Biology
Molecular Programming
Biochemistry

Background:

Catalytic DNA circuits are crucial for signal amplification in molecular programming.
Reactant recycling is essential for continuous operation in these circuits.
Improving recyclability in entropy-driven DNA circuits is a key challenge.

Purpose of the Study:

To develop and implement a novel nicking-assisted recycling strategy for reactants in entropy-driven DNA circuits.
To enhance the efficiency and recyclability of DNA circuits for molecular programming.
To investigate the performance of this strategy in both single-layered and two-layered circuit designs.

Main Methods:

Implementation of a nicking-assisted recycling strategy where DNA waste products revert to active components.
Construction and analysis of single-layered and multiple two-layered entropy-driven DNA circuits.
Evaluation of reactant recycling during fuel DNA digestion and trigger release.

Main Results:

The single-layered catalytic circuit effectively consumes excess fuel DNA without depleting gate components.
Recycling in two-layered circuits was observed during fuel DNA digestion but not during downstream trigger release.
Demonstrated the ability of duplex DNA waste products to revert into active components for subsequent reaction cycles.

Conclusions:

The nicking-assisted recycling strategy offers a simple and versatile method for improving entropy-driven DNA circuits.
This approach enhances the efficiency of DNA circuits for applications in molecular programming and synthetic biology.
The strategy facilitates the creation of more robust and sustainable catalytic DNA systems.