DNA Biosensor Based on Double-Layer Discharge for the Detection of HPV Type 16

Area of Science:

• Electrochemistry
• Biosensor Technology
• Molecular Diagnostics

Background:

• DNA electrochemical biosensors offer a promising diagnostic tool for various diseases.
• Accurate detection of Human Papillomavirus-16 (HPV-16) is crucial for diagnosing associated pathologies.

Purpose of the Study:

• To develop a novel electrochemical biosensor for the sensitive detection of HPV-16 DNA.
• To investigate potential relaxation measurements for DNA hybridization sensing.

Main Methods:

• Development of a DNA-modified gold electrode for HPV-16 sensing.
• Utilizing potential relaxation measurements and electrochemical impedance spectroscopy (EIS).
• Employing a rectifier diode arrangement to isolate double-layer current contributions.

Main Results:

• A unique equivalent circuit model for the DNA/Au electrode was proposed and validated.
• The biosensor achieved a detection limit of 0.38 nM for HPV-16 DNA sequences.
• Qualitative discrimination between complementary and mismatched HPV-16 DNA sequences was demonstrated.

Conclusions:

• The developed potential relaxation method is effective for HPV-16 DNA detection.
• This approach provides a viable strategy for creating advanced HPV DNA biosensors.
• The study highlights the potential of double-layer discharge detection in biosensing applications.