Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Peptide Bonds

Peptide Bonds

A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Traction Force Microscopy for Viscoelastic Substrates: A Semi-Analytical Method.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Restoration of structural organization in engineered cardiac microtissues is promoted by cardiomyocyte beating.

Communications biology·2026

Same author

Controlling the Physical Properties in Hybrid Hydrogel Networks via Tunable Supramolecular Interactions.

Macromolecules·2026

Same author

A noncanonical role for Jagged1 in endothelial mechanotransduction.

The FEBS journal·2026

Same author

Mechanosensitive interactions between Jag1 and Myo1c control Jag1 trafficking in endothelial cells.

iScience·2025

Same author

Macrophage Paracrine Signalling Differentially Affects Fibroblast-Induced Collagenous Tissue Remodelling.

Tissue engineering and regenerative medicine·2025

Search research articles

Related Experiment Video

Updated: Jan 19, 2026

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility

Published on: September 6, 2024

Functional peptide presentation on different hydrogen bonding biomaterials using supramolecular additives.

Ronald C van Gaal¹, Antonetta B C Buskermolen¹, Bastiaan D Ippel¹

¹Laboratory for Cell and Tissue Engineering, PO Box 513, 5600, MB, Eindhoven, the Netherlands; Institute for Complex Molecular Systems, PO Box 513, 5600, MB, Eindhoven, the Netherlands.

|September 23, 2019

Summary

Supramolecular biomaterials functionalized with cell adhesive peptides show distinct surface changes and cellular responses. The bisurea (BU) system, compared to ureido-pyrimidinone (UPy), more significantly influenced cell adhesion and migration behaviors.

Keywords:

Bisurea Focal adhesions Peptide presentation RGD Supramolecular biomaterials Ureido-pyrimidinone

More Related Videos

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Published on: August 15, 2016

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

Published on: February 27, 2019

Related Experiment Videos

Last Updated: Jan 19, 2026

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility

Published on: September 6, 2024

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

Published on: August 15, 2016

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

Published on: February 27, 2019

Area of Science:

Biomaterials Science
Supramolecular Chemistry
Cell Biology

Background:

Supramolecular biomaterials offer versatile functionalization via additive mixing.
The presentation and impact of bioactive additives in supramolecular elastomeric biomaterials remain underexplored.

Purpose of the Study:

To investigate how cell adhesive peptides (RGD and cRGD) are presented on ureido-pyrimidinone (UPy) and bisurea (BU) supramolecular biomaterials.
To evaluate the effects of these peptide-functionalized biomaterials on surface morphology and cellular behavior.

Main Methods:

Polycaprolactone modified with UPy or BU moieties was used as the base material.
Cell adhesive peptides (RGD, cRGD) were conjugated to complementary supramolecular motifs and incorporated as additives.
Surface morphology and cellular responses (focal adhesion, migration) were analyzed.

Main Results:

Bioactivation altered surface morphology, forming random aggregates on UPy and fibrous aggregates on BU materials.
Peptide type influenced aggregation; RGD induced larger clusters than cRGD.
Increased cRGD concentration reduced focal adhesion size and cell migration velocity, while increasing focal adhesion numbers, particularly on BU-biomaterials.

Conclusions:

UPy and BU supramolecular systems exhibit distinct peptide-presenting properties.
The BU-based system demonstrated a more pronounced effect on cellular adhesive behavior.
This study provides insights into peptide presentation in supramolecular elastomeric platforms for biomaterial applications.