Sonication-assisted Layer-by-Layer self-assembly nanoparticles for resveratrol delivery.

Area of Science:

• Nanotechnology
• Materials Science
• Pharmaceutical Sciences

Background:

• Resveratrol (RSV) has therapeutic potential but poor oral bioavailability due to low solubility.
• Conventional oral administration of RSV is limited by its physicochemical properties.
• Nanodelivery systems offer a promising strategy to overcome these limitations.

Purpose of the Study:

• To develop and characterize novel Layer-by-Layer (LbL) self-assembled nanoparticles for oral resveratrol (RSV) delivery.
• To investigate the effect of LbL bilayers on RSV nanoencapsulation and release kinetics.
• To evaluate the biopharmaceutical advantages of RSV LbL nanoformulations for oral administration.

Main Methods:

• Resveratrol (RSV) nanoprecipitation followed by washless Layer-by-Layer (LbL) self-assembly using poly(allylamine hydrochloride) (PAH) and dextran sulfate (DS).
• Fabrication of RSV nanoformulations with varying numbers of PAH/DS bilayers (2.5, 5.5, and 7.5).
• Characterization of nanoparticle size, polydispersity index, zeta potential, encapsulation efficiency, and cytocompatibility with Caco-2 cells.

Main Results:

• Developed RSV LbL nanoformulations with controlled nanoscale size (116-220 nm) and high encapsulation efficiency (>90%).
• Nanoformulations exhibited good colloidal and chemical stability, with excellent cytocompatibility.
• Layer-by-Layer nanoparticles demonstrated controlled release of RSV, particularly in simulated intestinal conditions, dependent on bilayer number.

Conclusions:

• Layer-by-Layer (LbL) self-assembly provides a viable strategy for nanoencapsulating poorly soluble resveratrol (RSV).
• PAH/DS LbL nanoparticles enhance the oral delivery potential of resveratrol by controlling its release.
• These findings suggest LbL nanoparticles are a promising nanodelivery system for expanding resveratrol's therapeutic applications.