Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Frequent Detection of Abasic Sites and <i>O</i><sup>6</sup>-Methyl-2'-Deoxyguanosine and Absence of Substituted Aromatic DNA Adducts in Colorectal Tissue of Smokers and Meat-Eaters with Colorectal Cancer.

Chemical research in toxicology·2026
Same author

Tissue-specific mutagenesis from endogenous guanine damage is suppressed by Polκ and DNA repair.

Nature communications·2025
Same author

The specificity and structure of DNA cross-linking by the gut bacterial genotoxin colibactin.

Science (New York, N.Y.)·2025
Same author

Untargeted DNA Adductomics Identifies Aristolochic Acid III as a Potent DNA-Damaging Agent among 11 Substituted Aromatic Genotoxicants in the Rat Urinary System.

Chemical research in toxicology·2025
Same author

The specificity and structure of DNA crosslinking by the gut bacterial genotoxin colibactin.

bioRxiv : the preprint server for biology·2025
Same author

Expanding the MR1 ligandome using chemical class-specific fragmentation and molecular networking.

bioRxiv : the preprint server for biology·2025
Same journal

Analytical Identification of Novel Furan Metabolites in Human Hepatocytes and Urine: Implications for Liver and Respiratory Disease Research.

Chemical research in toxicology·2026
Same journal

A Cross-Sectional Study on Immune-Inflammatory Responses: Exposure to Polycyclic Aromatic Hydrocarbons Alters Pteridine Metabolism and Activates the Kynurenine Pathway.

Chemical research in toxicology·2026
Same journal

ACSS2 Inhibition Alleviates Cisplatin-Induced Acute Kidney Injury: Insights from Targeted Metabolomics.

Chemical research in toxicology·2026
Same journal

AmesNet: A Task-Conditioned Deep Learning Model with Enhanced Sensitivity and Generalization in Ames Mutagenicity Prediction.

Chemical research in toxicology·2026
Same journal

DNA Structure-Dependent Enrichment of Oxidative Lesions.

Chemical research in toxicology·2026
Same journal

Characterizing the Reactive Metabolites of Colony-Stimulating Factor 1 Receptor Inhibitor PLX5622 in Liver Microsomes and Mice.

Chemical research in toxicology·2026
See all related articles

Related Experiment Video

Updated: Jan 19, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
09:33

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Published on: March 20, 2018

14.3K

Methods and Challenges for Computational Data Analysis for DNA Adductomics.

Scott J Walmsley1,2, Jingshu Guo1,3, Jinhua Wang1,2

  • 1Masonic Cancer Center , University of Minnesota , Minneapolis , Minnesota 55455 , United States.

Chemical Research in Toxicology
|September 25, 2019
PubMed
Summary
This summary is machine-generated.

DNA adductomics uses advanced mass spectrometry to screen for numerous DNA damages simultaneously. However, current software struggles to detect these low-abundance adducts, hindering cancer-causing agent identification.

More Related Videos

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

9.1K
Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications
10:12

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

3.6K

Related Experiment Videos

Last Updated: Jan 19, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
09:33

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Published on: March 20, 2018

14.3K
Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

9.1K
Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications
10:12

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

3.6K

Area of Science:

  • Environmental Health
  • Genomics
  • Analytical Chemistry

Background:

  • Chemicals in the environment, diet, and endogenous processes cause DNA adducts, which can lead to mutations and diseases like cancer.
  • Previous methods allowed targeted analysis of specific DNA adducts, but lacked the ability to screen for a comprehensive range of adducts in a single assay.
  • The human genome harbors diverse DNA damages, necessitating advanced analytical techniques for thorough characterization.

Purpose of the Study:

  • To introduce DNA adductomics as a novel, nontargeted approach for simultaneously screening multiple DNA adducts.
  • To discuss the limitations of current computational tools in processing mass spectrometry data for DNA adduct discovery.
  • To highlight the need for improved software and algorithms to advance DNA adductomics as a mature technology.

Main Methods:

  • Utilizing high-resolution mass spectrometry (MS) instrumentation and advanced scanning technologies.
  • Employing nontargeted "omics" approaches, including data-dependent and data-independent acquisition methods.
  • Evaluating contemporary computational tools for feature finding in MS data, commonly used in proteomics and metabolomics.

Main Results:

  • DNA adductomics enables the simultaneous screening of multiple DNA adducts, offering a broader view of genomic damage.
  • Existing software and common MS data acquisition methods show limitations in reliably detecting low-abundance DNA adducts in human samples.
  • Current computational tools, while effective in other omics fields, are not fully optimized for nontargeted DNA adduct biomarker discovery.

Conclusions:

  • DNA adductomics holds promise for identifying a wide spectrum of DNA damages and potential cancer-causing agents.
  • Significant challenges in data processing, particularly adduct detection at low abundance, must be addressed for DNA adductomics to reach its full potential.
  • Improvements in MS data processing software and the development of novel algorithms are crucial for establishing DNA adductomics as a powerful tool in environmental health and disease research.