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Updated: Jan 19, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Two Trifunctional Leloir Glycosyltransferases as Biocatalysts for Natural Products Glycodiversification
Ramesh Prasad Pandey, Puspalata Bashyal, Prakash Parajuli
1Genome-based BioIT Convergence Institute , 70 Sunmoon-ro 221, Tangjeong-myeon , Asan-si , Chungnam 31460 , Republic of Korea.
Abstract:
Two promiscuous Bacillus licheniformis glycosyltransferases, YdhE and YojK, exhibited prominent stereospecific but nonregiospecific glycosylation activity of 20 different classes of 59 structurally different natural and non-natural products. Both enzymes transferred various sugars at three nucleophilic groups (OH, NH2, SH) of diverse compounds to produce O-, N-, and S-glycosides. The enzymes also displayed a catalytic reversibility potential for a one-pot transglycosylation, thus bestowing a cost-effective application in biosynthesis of glycodiversified natural products in drug discovery.
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